Video Experimental Relacionado

Updated: Jan 13, 2026

Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology

Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology

Published on: August 18, 2014

Un papel de la 6-nitrodopamina derivada del epitelio en la contractilidad del uréter humano

Wilmar Azal Neto¹, Denis Lima Oliveira², José Britto-Júnior²

¹Department of Surgery, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, Brazil.

British journal of pharmacology

|January 7, 2026

Resumen

Los uréteres humanos liberan 6-nitrodopamina (6-ND), una novedosa catecolamina que influye en la contractilidad del músculo liso del uréter y modula la peristalsis ureteral.

Área de la Ciencia:

Urología
Farmacología
Fisiología

Sus antecedentes:

Las funciones fisiológicas del uréter humano no se comprenden completamente.
Las novedosas moléculas de señalización pueden desempeñar un papel en la contractilidad ureteral.

Objetivo del estudio:

Investigar la liberación basal de 6-nitrodopamina (6-ND) de uréteres humanos aislados.
Determinar el papel de la 6-ND en la contractilidad del músculo liso del uréter.

Principales métodos:

Se utilizaron segmentos de uréter humano de donantes de órganos.
La liberación basal de 6-ND se midió mediante cromatografía líquida-espectrometría de masas en tándem.
Las contracciones de anillos de uréter se midieron isométricamente y se analizó la expresión tisular de tirosina hidroxilasa (TH) y eNOS.

Principales resultados:

La liberación basal de 6-ND fue mayor en muestras de uréter intactas en comparación con las desnudas.
La 6-ND indujo contracciones dependientes de la concentración y potenció las contracciones inducidas por noradrenalina y adrenalina.
Se expresaron TH y eNOS en el epitelio ureteral.

Conclusiones:

El uréter humano libera 6-ND.
Esta novedosa catecolamina modula la contractilidad del músculo liso del uréter.
La 6-ND ofrece nuevas perspectivas sobre los mecanismos de la peristalsis ureteral.

Palabras clave:

CL–SM/SM adrenalina dopamina endotelial óxido nítrico sintasa nitro-catecolamina urotelio

Más Videos Relacionados

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

Published on: September 7, 2022

A Decentralized Ex Vivo Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling

A Decentralized Ex Vivo Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling

Published on: November 28, 2019

Videos de Conceptos Relacionados

Physiology of the Genitourinary System III: Urine Concentration and Dilution

Physiology of the Genitourinary System III: Urine Concentration and Dilution

The kidneys concentrate or dilute urine to maintain water and electrolyte balance. Nephrons, particularly the loop of Henle, play a crucial role in this process through the countercurrent multiplication system. This system establishes a high osmolarity in the renal medulla, which is essential for water reabsorption. In the loop of Henle’s descending limb, water is reabsorbed into the surrounding medulla due to its permeability to water. In contrast, the ascending limb actively transports...

Antihypertensive Drugs: Vasodilators

Antihypertensive Drugs: Vasodilators

Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...

Ureters

Ureters

The ureters are retroperitoneal tubes located on either side of the vertebral column. They are responsible for transporting urine from each kidney to the urinary bladder. These tubes have thick walls and are approximately 25-30 cm long. Their diameter is around 10 mm at the renal pelvis, gradually narrowing to 1 mm as the ureter obliquely enters the posterior bladder wall through the ureteric orifices. The shape of these orifices is slit-like, which helps to prevent urine backflow toward the...

Urethra

Urethra

The urethra is a hollowed tubular organ through which urine is expelled from the body. This structure extends from the bladder to the external opening, allowing urine to be released.
The anatomy of the urethra differs between males and females. In females, the urethra is short, measuring about 3–4 cm in length, and opens anterior to the vaginal opening. In males, the urethra is longer and passes through the penis, serving dual purposes: expelling urine and ejaculating semen. The male...

Anatomy of the Genitourinary System II: Bladder and Urethra

Anatomy of the Genitourinary System II: Bladder and Urethra

The lower urinary system consists of the urinary bladder and urethra, which are essential in storing and expelling urine from the body. Together with the internal and external sphincters, these structures work together to regulate urination effectively.Anatomy of the BladderThe urinary bladder is a muscular, stretchable organ behind the pubic bone and in front of the rectum. In females, the bladder is positioned anterior to the vagina and inferior to the uterus, while in males, it is located...

Introduction to Urinary System

Introduction to Urinary System

The urinary system consists of two kidneys, two ureters, the urinary bladder, and the urethra.
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...