Video Experimental Relacionado

Updated: Jan 13, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Una plataforma inercial-acústica para bioanálisis: separación concurrente, de alta precisión y alto rendimiento de

Zhuoyang Wang¹, Xianglian Liu¹, Junping Duan¹

¹State Key Laboratory of Extreme Environment Optoelectronic Dynamic Measurement Technology and Instrument, North University of China, Taiyuan, Shanxi, China.

Analytical and bioanalytical chemistry

|January 7, 2026

Abstract:

Efficient separation of cancer cells from whole blood is vital for early cancer diagnosis. However, disease progression often causes complications that reduce survival, such as cholangitis in liver cancer due to bile-duct compression, with Escherichia coli (E. coli) and Klebsiella as common pathogens. To address this, we developed a sheathless microfluidic sorter that integrates inertial focusing with surface acoustic wave (SAW) manipulation to simultaneously isolate cancer cells and bacteria. Specifically, we introduce a label-free, SAW-driven microfluidic platform that couples passive inertial focusing with active SAW manipulation to enable concurrent separation of cancer cells and bacteria; Cr/Au interdigital transducers (IDTs) are patterned on a 128° Y-cut LiNbO₃ substrate. The system first employs a contraction-expansion microchannel to achieve preliminary separation. Larger particles are then deflected by a slanted interdigital transducer (S-IDT), improving bacterial sorting purity. Small particles are enriched at the first outlet (purity > 96%), and the remaining targets are further refined by a focusing interdigital transducer (F-IDT). Tests with polystyrene (PS) particles achieved >96% purity, and in clinical samples, sorting efficiency and purity exceeded 93% for HepG2 cells and E. coli, with HepG2 viability above 94%. This compact platform unifies active and passive mechanisms, offering promise for early diagnosis and real-time monitoring of complications.

Palabras clave:

Acústica de fluidos Separación de células y bacterias Enfoque inercial Biopsia líquida de cáncer de hígado Clasificación microfluídica Ondas acústicas superficiales

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