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CmAP2 interactúa con CmRAX2 y promueve la ramificación al afectar la vía de las citoquininas en el crisantemo

Qin Ling¹, Peng Mei¹, Shaokang Tang¹

¹College of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan, China.

Plant physiology and biochemistry : PPB

|January 7, 2026

Resumen

Los investigadores identificaron una nueva proteína AP2, CmAP2, que regula positivamente la ramificación en el crisantemo interactuando con CmRAX2. La sobreexpresión de CmAP2 mejoró la ramificación y alteró las vías de las citoquininas, afectando los rasgos ornamentales.

Área de la Ciencia:

Biología Vegetal
Genética
Horticultura

Sus antecedentes:

La ramificación es un rasgo ornamental clave en el crisantemo (Chrysanthemum morifolium), que influye en el cultivo y la producción.
La familia de factores de transcripción APETALA2 (AP2) regula el crecimiento de las plantas, pero su papel en la ramificación no se comprende completamente.

Objetivo del estudio:

Identificar y caracterizar nuevos genes involucrados en la ramificación del crisantemo.
Elucidar el mecanismo molecular subyacente a la regulación de la ramificación mediada por CmAP2.

Principales métodos:

Se utilizaron ensayos de levadura de dos híbridos (Y2H), in vivo de complementación de fluorescencia bimolecular (BiFC), complementación dual de luciferasa (LCA) y co-inmunoprecipitación (Co-IP) para confirmar las interacciones proteicas.
Sobreexpresión heteróloga de CmAP2 en Arabidopsis thaliana y crisantemo.
Análisis de secuenciación de ARN (RNA-seq) de líneas de crisantemo transgénicas.

Principales resultados:

Se identificó una nueva proteína AP2, CmAP2, que interactúa con CmRAX2, un regulador positivo de la ramificación.
La sobreexpresión de CmAP2 en Arabidopsis y crisantemo promovió la ramificación, aumentó el número de yemas axilares y mejoró la altura de la planta.
La expresión de CmAP2 y CmRAX2 fue inducida por la decapitación, y el análisis de RNA-seq reveló una alteración en la expresión de genes relacionados con las citoquininas en las líneas transgénicas.

Conclusiones:

CmAP2 interactúa con CmRAX2 para regular positivamente la ramificación del crisantemo.
CmAP2 influye en la ramificación al modular las vías de biosíntesis y señalización de las citoquininas.
Este estudio proporciona información sobre la regulación genética de los rasgos ornamentales en el crisantemo.

Palabras clave:

Ramificación Crisantemo CmAP2 Citoquinina Interacción de proteínas

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