La transcriptómica desvela las ventajas de la técnica de compresión magnética en la reparación y regeneración de...

Magnetic compression technique (MCT) is a novel surgical operation leveraging the 'non-contact' magnetic forces to address challenging clinical issues. The underlying mechanisms of different healing efficacy in gastroenteric anastomosis are still not clarified between MCT and traditional suturing techniques.

A total of twenty male Sprague-Dawley (SD) rats, weighing 200-240g, were randomly and equally divided into traditional suturing (Con) and MCT group. The anastomosis construction time, survival rates, and postoperative complications were compared. Specimens were collected, and RNA sequencing was conducted at two weeks postoperatively, Meanwhile, the burst pressure and histological characteristics were compared between two groups.

The anastomosis time of MCT group (6.20 ± 1.51min) was significantly shorter than Con group (15.7 ± 2.43min; P < 0.001). Compared to Con group (80%), the survival rate was significantly higher in the MCT group (100%). No postoperative complications were observed in MCT group. The burst pressure and histological characteristics suggested the MCT group harbored more durable, smoother and fatter surface, complete mucosal migration, less inflammation infiltration, and continuous submucosal layers. The transcriptomics indicated that biological characteristic of MCT group was mainly enriched in energy metabolism, especially for lipid metabolism. The evaluated expression of Nfil3 and Pdk4 could serve as key metabolic molecules regulating anastomotic healing.

The MCT presented significant advantages in multiple dimensions, including simple, safety, low postoperative complications. A novel insight was provided that MCT facilitated energy metabolism and further promoted anastomotic healing.