Expresión génica placentaria basada en la paridad materna

Nulliparity is associated with adverse pregnancy outcomes including preeclampsia, preterm birth, lower birth weight and stillbirth although mechanisms are unclear. Placental gene expression differences, which also vary by fetal sex, may drive altered placental function and explain pregnancy outcome differences between nulliparous and multiparous women. This study aims to identify placental gene expression differences based on parity and examine their relationship with birth weight.

RNA sequencing was performed on placental samples collected at delivery as part of the NICHD Fetal Growth Studies. Differentially expressed genes between placentas of nulliparous (n = 34) and multiparous (n = 41) participants were determined in the full cohort and stratified by fetal sex. Weighted gene co-expression network analysis was performed to identify co-expression modules associated with parity. Correlation of differentially expressed genes and co-expression modules with birth weight was assessed.

Five differentially expressed genes were identified (FDR p < 0.05) including DDX5 (higher expression in multiparas in full cohort), ANKRD33 and SLITRK6 (higher in multiparas in female sub-cohort), and IL1B and MTCO1P40 (higher in nulliparas in female sub-cohort). Two gene co-expression modules, "Grey60" and "Tan", were negatively associated with multiparity. The DDX5 and ILB1 genes, and the "Grey60" module were significantly correlated with birth weight within the parity group that exhibited lower expression. Genes in the two co-expression modules were enriched for pathways related to immune response, cardiovascular and reproductive system development, and cancer.

Placental gene expression differences between nulliparas and multiparas may in part underlie neonatal outcomes that differ by parity and fetal sex.