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Updated: Jan 13, 2026

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Published on: May 17, 2020
TurboID: Etiquetado de Proximidad en Plantas
Chaonan Shi1, Huang Tan2, Rosa Lozano-Durán3
1Department of Plant Biochemistry, Centre for Plant Molecular Biology (ZMBP), Eberhard Karls University, Tübingen, Germany. chaonan.shi@uni-tuebingen.de.
Abstract:
Proximity labeling (PL) techniques have emerged as powerful approaches for the study of protein associations in vivo. In this chapter, we present a step-by-step TurboID-based PL protocol to define the "proxiome" of a protein of interest in plants, from the generation of the fusion protein to the analysis of the results, using the publicly available Ti-TAN plasmid collection.
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