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Protocolo para el establecimiento y caracterización morfológica de organoides cerebrales murinos cultivados a largo
Issam El-Debs1, Michael R Knittler2, Thomas C Mettenleiter3
1Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Abstract:
Murine cerebral organoids provide a rapid and reproducible in vitro system that recapitulates key aspects of neurogenesis. While human cerebral organoid protocols are well established, methods for non-human models remain limited. Here, we present a protocol for generating long-term cultured murine cerebral organoids from E14.5 embryonic stem cells (ESCs). We describe steps for generating mature organoids, followed by histological processing including paraffin embedding and microtome sectioning. We then detail procedures for characterizing murine cerebral organoids through H&E staining and immunofluorescence techniques.
