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Updated: Jan 13, 2026

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Protocolo para la generación de protoplastos del hongo simbiótico de la hormiga cortadora de hojas Leucoagaricus

Ayoub Stelate¹, Jonathan Z Shik²

¹Section for Ecology and Evolution, Department of Biology, University of Copenhagen, København, Denmark.

|January 10, 2026

Abstract:

We present a protocol to isolate viable protoplasted cells of the basidiomycete fungal mutualist (Leucoagaricus gongylophorus) obligately farmed by leafcutter ants. We describe steps for culturing actively growing mycelia, enzymatic digestion of fungal cell walls under osmotic stabilization, and purification of protoplasts (plasma membrane enclosing cytoplasm and organelles). We then detail procedures for assessing viability and wall removal using microscopy and fluorescent staining.

Palabras clave:

Biología Celular Aislamiento celular Ciencias Ambientales Biología Evolutiva Microscopía

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