Activación heterogénea de NF-κB y características de los potenciadores dan forma a la transcripción en la inmunidad...

Conserved NF-κB signaling pathways shape immune responses in animals. In mammals, NF-κB activation patterns and downstream transcription vary with stimulus, cell type, and stochastic differences among identically treated cells. Whether animals without adaptive immunity exhibit similar heterogeneity or rely on distinct immune strategies remains unknown. We engineered Drosophila melanogaster S2^∗ live reporter cells as an immune-responsive model to monitor the dynamics of an NF-κB transcription factor, Relish, and downstream transcription in single cells. Following immune stimulation, Relish exhibits diverse nuclear localization dynamics, with both the fraction of responsive cells and the speed of Relish activation increasing with stimulus dose. Pre-stimulus features, including the amount of nuclear Relish under basal conditions, predict a cell's responsiveness to stimulation. Simultaneous measurement of Relish and downstream transcription revealed that the probability of transcriptional bursts from immune-responsive enhancers correlates with Relish nuclear fraction. Enhancers containing more κB binding sites have a higher likelihood of activating at the population-level. Our study uncovers heterogeneity in NF-κB activation and target gene expression within Drosophila, illustrating how dynamic NF-κB behavior and enhancer architecture tune gene regulation.