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Sustrato compuesto de PTFE con nanocables ultraactivos para SERS para la detección de trazas de Aflatoxina B1 en agua
Liu Wang1, Jie Han1, Liangyan Sun1
1National Innovation Center for Digital Fishery, China Agricultural University, China; Key Laboratory of Smart Farming Technologies for Aquatic Animal and Livestock, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China; Beijing Engineering and Technology Research Centre for Internet of Things in Agriculture, China Agriculture University, Beijing 100083, China; College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
Abstract:
Aflatoxin B1 (AFB1), a potential carcinogen distributed in aquaculture systems, threatens aquatic health and food safety. However, current methods for detecting AFB1 in aquaculture water still rely on time-consuming sample pretreatment and costly large-scale instruments. Herein, we developed a high-performance surface-enhanced Raman scattering (SERS) sensor based on ultra-active silver nanowires (U-AgNWs) for AFB1 quantification in aquaculture water. PTFE substrate with hydrophobic condensation effect were selected to enhance SERS signal. Self-assembled AgNWs at the three-phase (oil-water-gas) interface were added onto the PTFE substrate. Subsequently, AgNWs were etched with aqua regia to increase surface roughness. Following iterative refinement, a novel PTFE substrate with remarkable SERS activity was successfully fabricated. This hybrid structure with significant SERS enhancement had been validated through FDTD simulation, and subsequent experiments had confirmed that the sensor had anti-interference, reproducibility, and repeatability. The sensor exhibited excellent linearity (R² > 0.97) for AFB1 within the range of 0.1ng/mL-104ng/mL and had satisfactory performance in aquaculture with recoveries of 97.3 %-103.2 % (reference HPLC/LM-MS). This sensor provides a simple, highly sensitive, and selective strategy for the timely detection of AFB1 in aquaculture, thus contributing to the security of aquatic products, the safeguarding of ecosystems, and the protection of public health. And this work also paved a way for developing facile devices to detect antibiotics, heavy metals, and other targets.
