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Updated: Jun 20, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Published on: October 15, 2013

Investigación de un pseudo-brote de Burkholderia cepacia complex causado por solución salina tamponada con fosfato

Anne Stone¹, Dawn Nolt², Rabeka Ali³

¹Division of Pediatric Pulmonology and Sleep Medicine, Department of Pediatrics, OHSU Doernbecher Children's Hospital, Portland, Oregon, United States.

American journal of infection control

|January 11, 2026

Abstracto

Introduction:

Burkholderia cepacia complex (BCC) is a cause of healthcare-associated infections in immunocompromised patients and people with cystic fibrosis (PwCF). This report summarizes a Burkholderia contaminans pseudo-outbreak related to improper laboratory processes affecting surgical specimens from immunocompetent individuals and respiratory cultures from PwCF.

Material And Methods:

In October 2024, an increase in BCC isolates involving surgical, respiratory, and urine specimens occurred at an academic tertiary hospital. An investigation was launched involving the electronic clinical surveillance system, environmental surveillance, and review of laboratory processes. Species identification and genotyping was performed with randomly amplified polymorphic DNA (RAPD) typing. Whole genome sequencing (WGS) was completed for clonality analysis.

Results:

A total of 19 BCC isolates were included, 17 collected for clinical indications and two from reagents during the investigation. BCC was recovered from phosphate-buffered saline (PBS) used for rehydrating surgical samples and Sputolysin (dithiothreitol) diluted with PBS and used to liquify respiratory samples. Species analysis revealed B. contaminans in surgical, respiratory, and reagent samples. RAPD typing and WGS confirmed clonality, suggesting that PBS was a source of extrinsic contamination.

Conclusions:

This investigation underscores the role of multidisciplinary collaboration, robust electronic health information systems, and microbial genomic tools in infection prevention and control, ensuring timely interventions and patient safety.

Palabras clave:

Burkholderia cepacia complex fibrosis quística solución salina tamponada con fosfato pseudo-brote secuenciación del genoma completo

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