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Quercetina-Fe nanodots inhibe la proliferación de células de cáncer de cuello uterino dirigiéndose a PCNA
Youwei Zhou1, Sheng Cheng2, Lei Shu2
1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No 218, Jixi Road, Hefei, 230022 China.
Objective:
The targets and active components that affect the prognosis of cervical cancer remain unclear.
Methods:
Three microarray datasets were collected for differentially expressed genes (DEGs) screening. The pathway, function, protein interaction and prognosis were analysed. The active components, related targets, the active ingredient-target gene network were conducted. The protein expression of the hub gene was validated through immunohistochemistry and Nanodrugs Que-Fe NDs were prepared and validated by in vitro cellular functionalization experiment.
Results:
162 upregulated and 145 downregulated DEGs were screened out, including 10 hub genes, which were mainly clustered in DNA replication, cell division, proliferation, and cycle. The results of active ingredient-target gene network showed that the traditional Chinese medicine quercetin has the potential therapeutic effect on cervical cancer via targeting PCNA. Experiments focused on cellular functionalization demonstrated that the synthesized Que-Fe nanodots (NDs) were capable of decreasing the levels of proliferating cell nuclear antigen (PCNA) expression and suppressing the in vitro proliferation of HeLa cells.
Conclusion:
The growth of cervical cancer HeLa cells was suppressed in vitro by Que-Fe NDs. This finding indicates a promising novel therapeutic target that warrants additional investigation.
Supplementary Information:
The online version contains supplementary material available at 10.1186/s12645-025-00353-y.
