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Updated: Jan 14, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

baal-nf identifica variantes disruptoras de motivos que disminuyen la afinidad de unión del factor de transcripción

Breeshey Roskams-Hieter^1,2, Øyvind Almelid³, Chris P Ponting⁴

¹Institute of Genetics and Cancer, MRC Human Genetics Unit, Western General Hospital, University of Edinburgh, Edinburgh, EH4 2XU, UK. b.j.roskams-hieter@sms.ed.ac.uk.

|January 12, 2026

Abstract:

Human traits vary in part due to genetically-determined change of transcription factor binding affinity within gene regulatory regions. However, few trait-causal variants or mechanisms are known. Here we propose 1,935 variants as strong candidates for causally altering human traits. We discover these through baal-nf which uses chromatin immunoprecipitation-sequencing data to identify allelic imbalance at heterozygous sites for affinity-concordant positions within transcription factor- and co-factor binding motifs. These allele-specific binding sites are evolutionarily conserved and enriched for trait and gene expression associations. baal-nf and these high-quality allele-specific binding sites allow trait variation due to altered transcription factor binding to be investigated.

Palabras clave:

unión específica de alelos desequilibrio alélico ChIP-sequencing motivos factores de transcripción

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