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Una comparación técnica de plataformas de transcriptómica espacial en seis tipos de cáncer
Sergi Cervilla1, Daniela Grases1, Elena Perez1
1Josep Carreras Leukaemia Research Institute (IJC), Badalona, Spain.
Genome biology
|January 12, 2026
Resumen
Este estudio compara cinco plataformas de transcriptómica espacial utilizando muestras de tumores FFPE, revelando fortalezas y limitaciones de las plataformas para la multiómica espacial e informando futuras estrategias de perfilado de tumores FFPE.
Área de la Ciencia:
- Genómica
- Biología Molecular
- Bioinformática
Sus antecedentes:
- Las tecnologías de transcriptómica espacial (ST) ofrecen nuevas perspectivas sobre la organización tisular y el contexto celular.
- Existe una evaluación técnica limitada de las plataformas ST, especialmente en muestras clínicas FFPE (fijadas en formalina e incrustadas en parafina).
Objetivo del estudio:
- Evaluar sistemáticamente cinco plataformas comerciales de ST utilizando secciones de tumores humanos FFPE coincidentes.
- Permitir comparaciones técnicas directas entre modalidades de captura espacial basadas en secuenciación e imagen.
- Evaluar el rendimiento de la plataforma para la integración de la multiómica espacial.
Principales métodos:
- Evaluación de cinco plataformas ST (Visium v1, v2/CytAssist, HD; Xenium; CosMx) en secciones de tumores humanos FFPE coincidentes de seis tipos de cáncer.
- Evaluación de la detección de transcritos y UMI, la concordancia gen-histología y la recuperación de tipos celulares.
- Integración con paneles de proteínas dirigidos y análisis de estrategias de muestreo y cobertura de área.
Principales resultados:
- Comparación directa de plataformas ST basadas en secuenciación e imagen en las mismas muestras FFPE.
- Primera comparación de las plataformas Xenium Multi-Tissue y Xenium Prime en la misma muestra.
- Demostración de las capacidades multiómicas espaciales y cuantificación del desacoplamiento ARN-proteína en tumores FFPE.
Conclusiones:
- Proporciona un conjunto de datos armonizado y una referencia técnica para la comunidad de transcriptómica espacial.
- Ofrece información sobre las fortalezas y limitaciones relativas de las plataformas ST para el perfilado de tumores FFPE.
- Guía las consideraciones de diseño para el perfilado espacial de alto rendimiento de tejidos FFPE.
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