Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

9.7K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
9.7K
pH Regulation in Cells01:28

pH Regulation in Cells

7.5K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
7.5K
Resting Potential Decay01:15

Resting Potential Decay

6.0K
The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl−) and highly permeable to potassium ions (K+). Differences in the ions' concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
At rest, the K+ is the main ion that moves across the membrane...
6.0K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

18.4K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
18.4K
Mitochondrial Membranes01:45

Mitochondrial Membranes

16.6K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
16.6K
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

16.7K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
16.7K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Imageomics defines granular morphological changes of human skin with age and reveals a rejuvenating effect of xenografting.

bioRxiv : the preprint server for biology·2026
Same author

Single-cell analyses identify independent aging processes that compete to determine cellular fate in budding yeast.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Genetic correlation-guided mega-analysis of DO mice provides mechanistic insight and candidate genes for age-related pathologies.

PLoS genetics·2026
Same author

Exploring a crossroads in the aging process.

eLife·2025
Same author

Loss of UXS1 Selectively Depletes Pyrimidines and Induces Replication Stress in KEAP1-Mutant Lung Cancer.

Cancer research·2025
Same author

Visualizing the internalization and biological impact of nanoplastics in live intestinal organoids by Fluorescence Lifetime Imaging Microscopy (FLIM).

Light, science & applications·2025
Same journal

Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

The Journal of cell biology·2026
Same journal

Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

The Journal of cell biology·2026
Same journal

Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

The Journal of cell biology·2026
Same journal

PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

The Journal of cell biology·2026
Same journal

Sequential changes in calcium transients during M phase regulate cardiomyocyte proliferation.

The Journal of cell biology·2026
Same journal

Role of the nonhelical tailpiece of myosin-II in regulating filament architecture and function.

The Journal of cell biology·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Jan 14, 2026

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess
07:35

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

Published on: June 1, 2022

2.6K

La homeostasis del ion potasio modula la función mitocondrial

Adam James Waite1, Beiduo Rao1, Elizabeth Schinski1

  • 1Calico Life Sciences LLC , South San Francisco, CA, USA.

The Journal of cell biology
|January 13, 2026
PubMed
Resumen
Este resumen es generado por máquina.

La reducción del potasio interno en las células aumenta el potencial de membrana mitocondrial (PMM) y extiende la vida útil. Este hallazgo sugiere que la modulación de los niveles de potasio podría ser una estrategia para un envejecimiento más saludable.

Palabras clave:
potasiomitocondriaenvejecimientopotencial de membrana mitocondrialvida útil

Más Videos Relacionados

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
05:27

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Published on: July 20, 2022

2.2K
Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics
07:03

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics

Published on: August 23, 2024

1.4K

Videos de Experimentos Relacionados

Last Updated: Jan 14, 2026

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess
07:35

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

Published on: June 1, 2022

2.6K
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
05:27

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Published on: July 20, 2022

2.2K
Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics
07:03

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics

Published on: August 23, 2024

1.4K

Área de la Ciencia:

  • Biología Celular
  • Investigación sobre el Envejecimiento
  • Biología Mitocondrial

Sus antecedentes:

  • La disminución del potencial de membrana mitocondrial (PMM) asociada con la edad se relaciona con el envejecimiento y las enfermedades relacionadas.
  • El papel causal de la disminución del PMM en el envejecimiento sigue sin estar claro, lo que cuestiona la viabilidad de las intervenciones dirigidas al PMM.

Objetivo del estudio:

  • Identificar factores genéticos que influyen en la disminución del PMM asociada con la edad utilizando una plataforma de cribado.
  • Investigar si la modulación del PMM puede afectar la vida útil y promover un envejecimiento más saludable.

Principales métodos:

  • Se desarrolló una plataforma de cribado en Saccharomyces cerevisiae (levadura) para identificar mutaciones que afectan el PMM.
  • Se caracterizó el mutante de vida más prolongada para comprender los mecanismos de mantenimiento del PMM y la extensión de la vida útil.
  • Se probaron intervenciones que incluyen la deleción de genes, la modulación de la actividad enzimática y la reducción del potasio ambiental.

Principales resultados:

  • Se identificaron mutaciones que ralentizaron o previnieron la disminución del PMM asociada con la edad.
  • Un mutante de vida más prolongada exhibió un PMM aumentado y una vida útil extendida debido a la reducción del potasio interno.
  • Intervenciones específicas, incluida la deleción de un transportador de potasio y la reducción del potasio ambiental, mejoraron el PMM celular y la vida útil.
  • En mitocondrias aisladas, la reducción de la concentración de potasio aumentó directamente el PMM.

Conclusiones:

  • Los niveles de potasio interno regulan críticamente la función mitocondrial y la vida útil celular.
  • La modulación del potasio intracelular es una estrategia viable para mejorar el potencial de membrana mitocondrial y promover un envejecimiento más saludable.