Video Experimental Relacionado

Updated: May 5, 2026

Moderate Prenatal Alcohol Exposure and Quantification of Social Behavior in Adult Rats

Moderate Prenatal Alcohol Exposure and Quantification of Social Behavior in Adult Rats

Published on: December 14, 2014

Las bebidas endulzadas con fructosa inducen desregulación redox diurna en MASLD pediátrica

Helaina E Huneault¹, Scott E Gillespie², Zachery R Jarrell³

¹Department of Pediatrics, Division of Gastroenterology, Hepatology, & Nutrition, Emory University, Atlanta, GA, USA; Department of Pediatrics and Human Development, Michigan State University, College of Human Medicine, Grand Rapids, MI, USA.

|January 14, 2026

Abstracto

Background:

Plasma glutathione/glutathione disulfide (GSH/GSSG) and cysteine/cystine (Cys/CySS) redox couples undergo diurnal variation in adults and are more oxidized in obesity-related conditions, including metabolic dysfunction-associated steatotic liver disease (MASLD). There is limited research on redox in children and no data on redox responses to sugars, despite high sugar consumption in this population. This study aimed to describe the diurnal variation of redox couples in children, assess the impact of MASLD, and evaluate responses to fructose versus glucose beverages.

Methods:

In a 2-day randomized, controlled, crossover feeding study, 26 children (12 with MASLD, 14 controls; aged 10-18 years) consumed isocaloric meals with fructose beverages (FB) on one day and glucose beverages (GB) (set as control) on another, following a washout period. Blood was collected every 2 h over 24 h and analyzed for Cys/CySS and GSH/GSSG. Redox potentials, E_h(Cys/CySS) and E_h(GSH/GSSG), were calculated using the Nernst equation. Linear mixed models assessed diurnal variation and effects of MASLD and beverage type.

Results:

Plasma E_h(GSH/GSSG) and E_h(CyS/CySS) varied significantly over time after both FB and GB (p < 0.05). With FB, E_h(GSH/GSSG) was significantly more oxidized in children with MASLD (p = 0.034); this was not observed with GB. Among children with MASLD, FB also led to greater E_h(GSH/GSSG) oxidation and lower GSH levels overnight (p < 0.05). While E_h(Cys/CySS) showed a similar trend, differences did not reach statistical significance.

Conclusions:

Our findings demonstrate that plasma redox states vary diurnally in children and are more oxidized in those with MASLD. Fructose intake increased oxidation of the GSH/GSSG redox couple and lowered GSH concentrations overnight, indicating heightened oxidative stress. These results identify fructose as a driver of redox imbalance in pediatric MASLD and support fructose reduction and glutathione restoration as therapeutic targets.

Palabras clave:

Azúcares dietéticos Glutatión Esteatosis hepática Estrés oxidativo

Más Videos Relacionados

Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats

Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats

Published on: August 24, 2016

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

Published on: May 13, 2022

Videos de Conceptos Relacionados

Glucose Transporters

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes: