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Updated: Jan 17, 2026

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Deep imaging by NIR-II multifocal structured illumination microscopy with enhanced super-resolution radial

Liang Feng, Jiaqi Li, Jie Zhang

|January 15, 2026

Resumen

New NIR-II MSIM-eSRRF microscopy combines techniques to overcome scattering and motion artifacts. This advanced deep imaging platform enhances resolution and contrast for studying live tissues, improving intravital microscopy.

Área de la Ciencia:

Imagen Biomédica
Microscopía Óptica
Microscopía de Super-resolución

Sus antecedentes:

La microscopía de iluminación estructurada multifocal (MSIM) en el segundo infrarrojo cercano (NIR-II) ofrece una resolución y profundidad mejoradas para especímenes turbios.
Sin embargo, el ruido inducido por la dispersión y los artefactos de movimiento limitan su aplicación en la imagen de tejidos vivos.

Objetivo del estudio:

Desarrollar una técnica de microscopía novedosa que mejore las capacidades de imagen profunda en tejidos biológicos dispersos.
Superar las limitaciones del ruido de fondo y los artefactos de movimiento en NIR-II MSIM.

Principales métodos:

Integración de la fluctuación radial super-resolutiva mejorada (eSRRF) con NIR-II MSIM.
Prueba de la plataforma híbrida NIR-II MSIM-eSRRF en fantasmas de Intralipid, tejido de cerdo ex-vivo y ratones vivos.

Principales resultados:

NIR-II MSIM-eSRRF demostró una mejora en la resolución espacial y el contraste en especímenes turbios.
La técnica suprimió eficazmente los artefactos de movimiento, permitiendo la visualización sin artefactos de la vasculatura cerebral en ratones vivos.
Se logró un rendimiento mejorado de imagen profunda en entornos de dispersión desafiantes.

Conclusiones:

NIR-II MSIM-eSRRF es una herramienta poderosa para la imagen profunda en entornos de alta dispersión.
Esta plataforma avanza la microscopía de fluorescencia intravital para el estudio de procesos fisiológicos.
La técnica es prometedora para acelerar los descubrimientos en la investigación de imágenes de tejidos vivos.

Palabras clave:

microscopía imagenología resolución tejidos artefactos NIR-II MSIM eSRRF fluorescencia intravital

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