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Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System

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Establecimiento de una línea de células madre embrionarias humanas con deleción de GPD1L (WAe009-A-80) utilizando el

Tao Dong¹, Yan Zhao², Hai-Feng Jin¹

¹Department of Anatomy, Histology and Embryology, Basic Medicine School, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China; Heilongjiang Provincial Key Laboratory of Food & Medicine Homology and Metabolic Disease Prevention, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China.

Stem cell research

|January 15, 2026

Abstract:

The GPD1L gene is located on 3p22.3. It encodes the glycerol phosphate dehydrogenase 1-like protein with homology to glycerol phosphate dehydrogenase (GPD1L), but the function of this enzyme is unclear. Mutations in GPD1L have been associated with BrS (Brugada syndrome) and SIDS (sudden infant death syndrome) and reduce Na⁺ inward current through an unknown mechanism in human cardiomyocytes. Here, a GPD1L knockout human embryonic stem cell line was generated using CRISPR/Cas9 system. The GPD1L knockout human embryonic stem cell maintains the pluripotency, differentiation into three germ layers, forming normal EBs.

Palabras clave:

genética células madre embrionarias humanas CRISPR/Cas9 deleción de GPD1L síndrome de Brugada

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