Video Experimental Relacionado

Updated: Jan 18, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

Un ensamblaje genómico de telómero a telómero para Cyperus difformis

Jingbo Li¹, Jian Zhao², Wei Zheng³

¹Engineering Research Center for Salt- and Alkali-Tolerant Food Crop Seeds of Guangxi Zhuang Autonomous Region, Guangxi Vocational University of Agriculture, Nanning, 530000, China.

Scientific data

|January 15, 2026

Resumen

Se generó un ensamblaje genómico de alta calidad para Cyperus difformis, una maleza problemática del arroz. Este recurso ayuda a comprender los mecanismos de resistencia a herbicidas y la evolución de C. difformis.

Área de la Ciencia:

Genómica
Ciencia de las Plantas
Ciencia Agrícola

Sus antecedentes:

Cyperus difformis es una maleza importante en el cultivo de arroz, que causa pérdidas significativas de rendimiento.
La rápida evolución de la resistencia a herbicidas en C. difformis complica las estrategias de manejo de malezas.

Objetivo del estudio:

Generar un ensamblaje genómico de telómero a telómero de alta calidad de Cyperus difformis.
Proporcionar un recurso genómico fundamental para comprender la resistencia a herbicidas y la biología de C. difformis.

Principales métodos:

Se utilizó una combinación de lecturas cortas PacBio HiFi, Oxford Nanopore Technologies (ONT), MGI y captura de conformación de cromatina de alto rendimiento (Hi-C).
Se anclaron los contigs a los cromosomas utilizando datos de Hi-C y se definieron los telómeros.

Principales resultados:

Se ensambló un genoma de 226 Mb con un N50 de andamio de 13,08 Mb.
Se anclaron el 97,24% de los contigs a 18 cromosomas y se identificaron 35 telómeros.
Se anotaron 19.347 genes codificantes de proteínas (91,8%) y se caracterizaron secuencias repetitivas.

Conclusiones:

El ensamblaje genómico de telómero a telómero de C. difformis es un recurso valioso.
Facilita la investigación en genética de poblaciones, genómica comparativa y la evolución de la resistencia a herbicidas en esta especie de maleza clave.

Palabras clave:

Cyperus difformis genoma ensamblaje resistencia a herbicidas malezas de arroz genómica evolución

Más Videos Relacionados

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

Published on: August 4, 2016

Videos de Conceptos Relacionados

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Telomeres and Telomerase

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...

Assembly of Cytoskeletal Filaments

Assembly of Cytoskeletal Filaments

Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...