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Updated: Jul 9, 2026

Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

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Published on: April 24, 2013

Protocolo para el uso de un ensayo ELISA para detectar los niveles totales de α-sinucleína en líneas de Drosophila

Marco Sciortino, Raquel Velazquez, Haven Tillmon

bioRxiv : the preprint server for biology

|January 16, 2026

Abstract:

In Parkinson's disease (PD), alpha-synuclein (α-syn) aggregation causes neuronal dysfunction and death, particularly of dopaminergic neurons, which is central to PD symptoms and progression. This protocol describes the use of a sandwich ELISA assay to quantify total α-syn levels in various Drosophila melanogaster genotypes expressing human α-syn point mutations associated with PD. Utilizing this protocol allows for the precise quantification of total α-syn levels in the Drosophila melanogaster (fly) model.

Palabras clave:

ensayo ELISA alfa-sinucleína Drosophila melanogaster Parkinson protocolo

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