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Updated: Jan 18, 2026
Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease
Published on: November 8, 2017
Permeabilidad diferencial de la barrera hematoencefálica impulsada por hipoperfusión asimétrica en un modelo de
Background:
Blood-brain barrier (BBB) dysfunction has been associated with vascular dementia (VaD). However, the underlying mechanisms causing BBB dysfunction remain unclear, especially regarding cerebral hypoperfusion. This study aims to investigate the effects of asymmetric hypoperfusion on BBB permeability using a mouse model of VaD.
Methods:
Asymmetric bilateral carotid artery stenosis (ACAS) was induced using ligature rings. BBB integrity was assessed on 3 days post-surgery using Evans blue, IgG and albumin extravasation. A modified tissue processing protocol optimized endogenous markers detection. Regional and sex-based differences in BBB permeability were analyzed.
Results:
Increased BBB permeability was observed in both corpus callosum and cortical areas, with significantly more severe leakage in the left cortex compared to the right, correlating directly with lower cerebral blood flow on the left side. In contrast, no significant asymmetrical difference in BBB permeability was detected in the corpus callosum. Additionally, male and female mice exhibited similar patterns of BBB permeability.
Conclusion:
This study provides the first evidence that asymmetrical hypoperfusion significantly impacts regional BBB permeability in a mouse model of VaD. These findings highlight the importance of local hemodynamic changes in driving BBB dysfunction and underscore potential therapeutic targets for preventing or mitigating vascular dementia progression.
