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Una microneedle activa de SERS para detectar la capacidad oxidativa y el potencial redox de ratas con estrés inducido
Jiajia Wang1, Lingling Ji2, Yuyao Zhang3
1State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 211189, China; Department of Acupuncture-Moxibustion, Massage and Rehabilitation, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Abstract:
To detect oxidative capacity and redox potential of organism simultaneously, a surface-enhanced Raman scattering-active microneedle was fabricated by integrating oxidative capacity and redox potential surface-enhanced Raman scattering probes in two grooves on an acupuncture needle, respectively. Different redox pairs solutions were used to assess the detection feasibility of the surface-enhanced Raman scattering-active microneedles. Oxidative capacity surface-enhanced Raman scattering probes, based on p-phenylenediamine, can discriminate minor changes in oxidative capacity, and redox potential surface-enhanced Raman scattering probes, based on an anthraquinone derivative, distinguish changes in redox potential of different redox pairs. Rats with or without high glucose-induced stress were utilized to evaluate the detection feasibility of the surface-enhanced Raman scattering-active microneedles in vivo. In different tissues, the two parameters show slight discrepancies. In the muscles of rats with high glucose-induced stress, no correlation was observed among oxidative capacity, redox potential, and blood glucose levels. The surface-enhanced Raman scattering-active microneedles would be a versatile tool for researches of oxidative capacity and redox potential in physiological and pathological processes.
