Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

17.8K
This article describes a method to obtain a three-dimensional (3D) structure of helically assembled molecules using cryo-electron microscopy. In this protocol, we use HIV-1 capsid assemblies to illustrate the detailed 3D reconstruction procedure for achieving a density map by the iterative helical real-space reconstruction...
17.8K
Microcrystal Electron Diffraction of Small Molecules09:48

Microcrystal Electron Diffraction of Small Molecules

7.2K
Here, we describe the procedures developed in our laboratory for preparing powders of small molecule crystals for microcrystal electron diffraction (MicroED) experiments.
7.2K
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

6.2K
We present the use of the MeshAndCollect protocol to obtain a complete diffraction data set, for use in subsequent structure determination, composed of partial diffraction data sets collected from many small crystals of the fluorescent protein...
6.2K
Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging07:29

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

42.0K
The protocol describes a high-throughput approach to determining structures of membrane proteins using cryo-electron tomography and 3D image processing. It covers the details of specimen preparation, data collection, data processing and interpretation, and concludes with the production of a representative target for the approach, the HIV-1 Envelope glycoprotein. These computational procedures are designed in a way that enables researchers and students to work remotely and contribute to data...
42.0K
Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

14.5K
We describe a correlative microscopy method that combines high-speed 3D live-cell fluorescent light microscopy and high-resolution cryo-electron tomography. We demonstrate the capability of the correlative method by imaging dynamic, small HIV-1 particles interacting with host HeLa...
14.5K
Real-Time Imaging of Bonding in 3D-Printed Layers04:36

Real-Time Imaging of Bonding in 3D-Printed Layers

3.9K
With a non-invasive and real-time technique, nanoscopic polymer motion inside a polymer filament is imaged during 3D printing. Fine-tuning this motion is crucial for producing constructs with optimal performance and appearance. This method reaches the core of plastic layer fusion, thus offering insights into optimal printing conditions and material design...
3.9K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Sub-Ångström Three-Dimensional Electron Diffraction Reveals Crystal Structures and Phase Transformations in Liquids.

Journal of the American Chemical Society·2026
Same author

<i>pyDiSCaMB</i>: enabling the use of multipolar scattering factors in <i>Phenix</i>.

Journal of applied crystallography·2026
Same author

Quantum refinement with electron diffraction and X-ray free-electron laser data: comparative study of ribonucleotide reductase dimetal site.

Journal of applied crystallography·2026
Same author

Automated Nanocrystalline Sponge Workflow Enabled by 3D Electron Diffraction.

Journal of the American Chemical Society·2026
Same author

Round robin on structure analysis from 3D electron diffraction data.

IUCrJ·2026
Same author

AutoLEI: An XDS-based pipeline with graphical user interface for automated real-time and offline batch 3D ED/microED data processing.

IUCrJ·2025
Same journal

Quantitative analysis of light-induced ion segregation in mixed-halide perovskites.

Journal of applied crystallography·2026
Same journal

Towards machine-learning-based on-the-fly analysis of neutron reflectometry.

Journal of applied crystallography·2026
Same journal

<i>mcstas_gisans</i>: combining ray tracing with the distorted-wave Born approximation using <i>McStas</i> and <i>BornAgain</i> for virtual GISANS experiments.

Journal of applied crystallography·2026
Same journal

Computational methods for automated center determination in electron diffraction patterns.

Journal of applied crystallography·2026
Same journal

Epitaxy of ultrathin Fe<sub>3</sub>O<sub>4</sub> films on SrTiO<sub>3</sub>(001): influence of growth parameters on the formation of coexisting (111)- and (001)-oriented phases.

Journal of applied crystallography·2026
Same journal

Spin excitations near the pressure-induced antiferromagnetic transition in SrCu<sub>2</sub>(BO<sub>3</sub>)<sub>2</sub>.

Journal of applied crystallography·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Jan 20, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.8K

Solución automatizada y en tiempo real de estructuras mediante difracción electrónica 3D

Yi Luo1,2, Yuwei Deng1, Bin Wang2

  • 1State Key Laboratory of Green Chemical Engineering and Industrial Catalysis, Sinopec Shanghai Research Institute of Petrochemical Technology, 1658 Pudong Beilu, Shanghai, 201208, People's Republic of China.

Journal of applied crystallography
|January 19, 2026
PubMed
Resumen
Este resumen es generado por máquina.

Instamatic-solve es un nuevo pipeline automatizado para la solución de estructuras cristalinas en tiempo real mediante difracción electrónica tridimensional (3D ED). Esta herramienta acelera significativamente el proceso, permitiendo el análisis rápido de diversos materiales.

Palabras clave:
difracción electrónica 3DInstamatic-solvesolución automatizada de estructuras

Más Videos Relacionados

Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

7.2K
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

6.2K

Videos de Experimentos Relacionados

Last Updated: Jan 20, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.8K
Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

7.2K
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

6.2K

Área de la Ciencia:

  • Ciencia de Materiales
  • Cristalografía
  • Química

Sus antecedentes:

  • La difracción electrónica tridimensional (3D ED) es crucial para el análisis de cristales a nanoescala.
  • La solución de estructuras 3D ED actual requiere mucha mano de obra, experiencia y carece de retroalimentación en tiempo real, lo que dificulta su adopción generalizada.

Objetivo del estudio:

  • Desarrollar un pipeline completamente automatizado y en tiempo real para la solución de estructuras 3D ED.
  • Optimizar el procesamiento de datos y la solución de estructuras, proporcionando información inmediata sobre la calidad y la estructura.

Principales métodos:

  • Implementación del pipeline Instamatic-solve en un microscopio electrónico de transmisión JEOL JEM 2100.
  • Procesamiento automatizado de datos de 3D ED para la determinación de estructuras en tiempo real.
  • Validación del pipeline en diversos materiales cristalinos, incluyendo zeolitas, híbridos y moléculas orgánicas.

Principales resultados:

  • Solución de estructuras cristalinas exitosa y rápida para diversos materiales en menos de 2 minutos.
  • El pipeline maneja datos de 3D ED en tiempo real y fuera de línea de diferentes plataformas.
  • El éxito de la solución de estructuras depende de la calidad de los datos, requiriendo una completitud ≥50% y una resolución <1.0 Å.

Conclusiones:

  • Instamatic-solve permite la solución de estructuras de materiales cristalinos de manera eficiente, automatizada y en tiempo real.
  • El pipeline democratiza la 3D ED al reducir la dependencia de conocimientos especializados.
  • Este avance tiene amplias implicaciones en múltiples disciplinas científicas.