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Super-resolution Imaging of the Bacterial Division Machinery08:47

Super-resolution Imaging of the Bacterial Division Machinery

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We describe a super-resolution imaging method to probe the structural organization of the bacterial FtsZ-ring, an essential apparatus for cell division. This method is based on quantitative analyses of photoactivated localization microscopy (PALM) images and can be applied to other bacterial cytoskeletal...
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We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution...
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Here, we describe a protocol for ultrasound localization microscopy (ULM), which achieves 12.5 µm spatial resolution to image the brain microvasculature in rats. It enables detailed visualization of blood flow direction and velocity, offering a powerful tool for advancing studies of cerebral circulation and vascular...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Test Samples for Optimizing STORM Super-Resolution Microscopy16:52

Test Samples for Optimizing STORM Super-Resolution Microscopy

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We describe the preparation of three test samples and how they can be used to optimize and assess the performance of STORM microscopes. Using these examples we show how to acquire raw data and then process it to acquire super-resolution images in cells of approximately 30-50 nm...
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Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging10:01

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The use of a hyperlens has been regarded as a novel super-resolution imaging technique due to its advantages in real-time imaging and its simple implementation with conventional optics. Here, we present a protocol describing the fabrication and imaging applications of a spherical...
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Video Experimental Relacionado

Updated: Jan 20, 2026

Super-resolution Imaging of the Bacterial Division Machinery
08:47

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Super-resolución de RM de cabeza anisotrópica a través del plano de coherencia de ciclo y sin necesidad de ejemplos

Samuel W Remedios1, Shuwen Wei1, Aaron Carass1

  • 1The Image Analysis and Communications Laboratory, Johns Hopkins University, Baltimore, USA.

Information processing in medical imaging : proceedings of the ... conference
|January 19, 2026
PubMed
Resumen

Este estudio presenta un novedoso modelo de espacio nulo de difusión de eliminación de ruido (DDNM) para la super-resolución (SR) de imágenes de resonancia magnética (RM). El método garantiza imágenes de RM realistas y de alta resolución con coherencia de ciclo, mejorando la resolución a través del plano en escaneos anisotrópicos.

Palabras clave:
RMcoherencia de ciclomodelo generativosuper-resoluciónsin necesidad de ejemplos

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