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Análisis Metabólico en Chip Utilizando Tecnología RMN de Banco

Marc Azagra1, Hetal Patel2, Alejandro Portela1

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Desarrollamos un novedoso espectrómetro de Resonancia Magnética Nuclear (RMN) de banco para la monitorización metabólica en tiempo real de sistemas de órgano en chip. Este avance permite el análisis no invasivo del metabolismo celular dentro de plataformas microfluídicas.

Palabras clave:
Resonancia Magnética Nuclearórgano en chipmetabolismomicrofluídicaanálisis en tiempo realcultivo celularbiotecnologíaquímica analíticabiología celular

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Área de la Ciencia:

  • Biotecnología
  • Química Analítica
  • Biología Celular

Sus antecedentes:

  • Los sistemas de órgano en chip (OoC) son modelos in vitro avanzados, pero carecen de herramientas de monitorización metabólica en tiempo real y no invasivas.
  • La espectroscopía de Resonancia Magnética Nuclear (RMN) es una técnica potente para el análisis metabólico, sin embargo, las configuraciones actuales son incompatibles con los formatos de chip microfluídico.

Objetivo del estudio:

  • Presentar el primer espectrómetro RMN de banco diseñado para la monitorización metabólica en tiempo real de cultivos celulares en plataformas microfluídicas.
  • Adaptar la tecnología RMN existente para una integración perfecta con sistemas de órgano en chip.

Principales métodos:

  • Se utilizó la hiperpolarización mediante polarización nuclear dinámica por disolución (dDNP) para mejorar la sensibilidad de la señal de RMN.
  • Se modificó un espectrómetro RMN de banco comercial y se diseñó una plataforma microfluídica personalizada para la inyección precisa de sustratos y la renovación del medio.
  • Se integró una bobina de radiofrecuencia y un portamuestras personalizado para la transmisión y recepción de señales.

Principales resultados:

  • Se demostró la viabilidad de la monitorización metabólica en tiempo real en plataformas microfluídicas utilizando el sistema desarrollado.
  • Se adquirieron datos preliminares de RMN que muestran estudios metabólicos dinámicos dentro de la configuración integrada.

Conclusiones:

  • El espectrómetro RMN de banco desarrollado representa un avance significativo para el análisis metabólico no invasivo en la investigación de órgano en chip.
  • Esta tecnología facilita estudios metabólicos dinámicos, mejorando la relevancia fisiológica y las capacidades analíticas de los modelos de cultivo celular microfluídico.