Video Experimental Relacionado

Updated: Jan 21, 2026

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized ¹³C NMR

Published on: July 8, 2025

Análisis Metabólico en Chip Utilizando Tecnología RMN de Banco

Marc Azagra¹, Hetal Patel², Alejandro Portela¹

¹Institute for Bioengineering of Catalonia, Barcelona Institute of Science and Technology, 08028 Barcelona, Spain.

Analytical chemistry

|January 19, 2026

Abstract:

Organ-on-chip (OoC) systems are advancing rapidly as physiologically relevant in vitro models. However, real-time, noninvasive metabolic monitoring tools remain lacking. While NMR offers a powerful solution, current setups are not compatible with the planar format of microfluidic chips. Here we introduce the first benchtop NMR spectrometer for real-time metabolic monitoring of cell cultures on microfluidic platforms, utilizing hyperpolarization via dissolution dynamic nuclear polarization. This work details modifications made to a commercial benchtop NMR spectrometer, including the design and fabrication of a microfluidic platform that enables precise injection of hyperpolarized substrates and continuous renewal of cell culture media. The platform integrates a radiofrequency coil for signal transmission and reception and incorporates a custom-built sample carrier. Preliminary NMR data acquired with this system demonstrate its feasibility for dynamic metabolic studies.

Palabras clave:

Resonancia Magnética Nuclear órgano en chip metabolismo microfluídica análisis en tiempo real cultivo celular biotecnología química analítica biología celular

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