Video Experimental Relacionado

Updated: Jan 22, 2026

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Published on: April 13, 2018

Propolis de Sonoran induce un efecto proapoptótico a través de la detención mitótica: un resultado distinto del ciclo

Efrain Alday¹, Patricia Ruiz-Bustos¹, Adriana Garibay-Escobar¹

¹Department of Chemistry-Biology, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora 83000, Mexico.

The Journal of pharmacy and pharmacology

|January 21, 2026

Abstracto

Objectives:

The aim of this study was to elucidate the molecular events of cell cycle progression underlying alterations in cell morphology, as well as the pro-apoptotic and antiproliferative effects of Sonoran propolis (SP) on cancer cells.

Methods:

Cell cycle progression was analysed in human (HeLa) and murine (M12.C3.F3) cancer cell lines treated with SP, its primarily plant source Populus fremontii resins (PFR), and other potential contributing plants [Bursera laxiflora, Ambrosia ambrosioides, and Ambrosia confertiflora (ACR)]. In addition, bio-guided fractionation was performed to identify potential minor bioactive phytochemicals involved and their botanical sources.

Key Findings:

SP (12.5 μg/ml) and ACR (3 μg/ml) induced mitotic arrest in both cancer cell models, characterized by an increase in elongated cyclin B1-positive cells, displaying alterations in nuclear and cytoskeleton organization, indicative of a defective spindle assembly checkpoint during metaphase. In contrast, PFR (12.5 μg/ml) increased cell population at G0/G1 in both cell lines, in comparison to dimethyl sulfoxide control. SP8020-XIV-7 chromatographic fraction induced G2/M arrest in treated cells, and Ultra-High Performance Liquid Chromatography-Quadrupole Time-Of-Flight Mass Spectrometry (UPLC-ESI-Q-TOF-HD-MS/MS) analysis revealed that it was composed by naringenin (<1%), kaempferol (23%), kaempferol-3-methyl ether (75%), and an unknown compound [(M-H)-: 343.0817 m/z, C18H16O7] (<2%), structurally related to eupatorin (G2/M inducer), which was additionally identified in ACR.

Conclusions:

We found that phytochemicals from A. confertiflora may contribute to SP bioactivity on cancer cells.

Palabras clave:

Ambrosia confertiflora DC Populus fremontii S. Watson flavonoides metoxilados antiproliferativos disfunción mitótica propolis tipo álamo propolis semiárido

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