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Updated: Jan 23, 2026
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Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice
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Una estrategia para el análisis de mezclas de fluidos corporales utilizando tDMRs específicos de semen y epidermis y
Sang Un Park1, Bo Min Kim1, Soyi Park1
1Department of Forensic Medicine, Seoul National University College of Medicine, Seoul, Korea.
Forensic science international. Genetics
|January 21, 2026
Resumen
El análisis forense de mezclas de ADN de semen o epidermis es un desafío. Un nuevo ensayo que combina CpGs metilados y microhaplotipos (MH) distingue eficazmente el ADN de múltiples contribuyentes en muestras forenses.
Área de la Ciencia:
- Ciencia Forense
- Genética
- Biología Molecular
Sus antecedentes:
- El análisis forense de mezclas de ADN de semen o epidermis es un desafío, especialmente con proporciones similares de múltiples contribuyentes.
- La identificación de donantes individuales a partir de muestras de fluidos corporales mixtos sigue siendo un obstáculo importante en las investigaciones forenses.
Objetivo del estudio:
- Desarrollar y validar un novedoso ensayo combinado de marcadores genéticos para mejorar el perfil de ADN específico de tejido a partir de mezclas forenses complejas.
- Superar las limitaciones en el análisis de ADN de semen o epidermis en mezclas con proporciones similares de contribuyentes.
Principales métodos:
- Identificación de CpGs metilados específicos de semen o epidermis utilizando datos de matrices de metilación de ADN.
- Selección de microhaplotipos (MH) o polimorfismos de nucleótido único (SNPs) cercanos utilizando bases de datos públicas y muestras de ADN coreanas.
- Desarrollo de un ensayo combinado CpG-MH y su aplicación mediante secuenciación masiva paralela (MPS) en muestras forenses de fuente única y mixtas.
Principales resultados:
- Se seleccionaron un total de 21 loci CpG-MH, incluyendo 16 CpGs metilados específicos de semen y cinco específicos de epidermis.
- El ensayo desarrollado excluyó eficazmente los alelos de tejido no objetivo en mezclas 1:1, produciendo predominantemente alelos de tejido objetivo.
- El ensayo demostró la capacidad de extraer información valiosa de mezclas con proporciones similares de dos contribuyentes.
Conclusiones:
- El novedoso ensayo CpG-MH permite un perfilado de ADN específico de tejido eficaz a partir de mezclas forenses desafiantes, incluyendo semen y epidermis.
- Este método mejora significativamente la interpretación de perfiles de ADN mixtos que antes eran difíciles de analizar.
- Se necesita un mayor desarrollo con modelos más sofisticados para mejorar el poder de discriminación individual en aplicaciones forenses.
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