Video Experimental Relacionado

Updated: Jan 23, 2026

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

Published on: September 5, 2017

La rutina exhibe actividad anticoagulante in vitro en muestras de sangre humana al prolongar los tiempos de la vía de

Anjan Palikhey¹, Laxmi Zaiswal², Amit Kumar Shrivastava¹

¹Department of Pharmacology, Universal College of Medical Sciences, Bhairahawa, Nepal.

Global advances in integrative medicine and health

|January 22, 2026

Abstracto

Background:

Rutin is a plant-derived flavonoid with reported biological activities, but its effect on blood coagulation parameters has not been clearly characterized under in-vitro conditions.

Objective:

This study aimed to evaluate the in-vitro anticoagulant activity of rutin in human blood by measuring changes in clotting time (CT), prothrombin time (PT), and activated partial thromboplastin time (aPTT) at increasing concentrations, compared with baseline control values.

Materials And Methods:

Rutin (1-6 mg/mL) was added to human blood samples, and standard CT, PT, and aPTT assays were used to measure coagulation parameters. Heparin and Phosphate-buffered saline (PBS) were used as controls.

Results:

In a concentration-dependent manner, rutin significantly increased activated partial thromboplastin time (aPTT), prothrombin time (PT), and clotting time (CT) compared with the PBS control, with the most pronounced effects observed at higher concentrations (4 and 6 mg/mL; P < 0.05 vs control). At 6 mg/mL, CT increased to 11.42 ± 1.11 minutes, PT to 21.33 ± 3.28 seconds, and aPTT to 57.20 ± 9.31 seconds.

Conclusion:

Rutin exhibits significant in vitro anticoagulant activity, supporting its potential as a natural anticoagulant candidate.

Palabras clave:

tiempo de tromboplastina parcial activada actividad anticoagulante flavonoide tiempo de protrombina rutina

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