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Estrategia multi-paso para el estudio de la tripa, un subproducto de la cerveza, utilizando LC-MS/MS y redes
Paolo Scognamiglio1, Vittorio Carlucci2, Nadia Benedetto2
1TheBlueChemistryLab Group, Department of Pharmacy, Università degli Studi di Napoli Federico II, Napoli, Italy.
Food chemistry
|January 22, 2026
Resumen
La tripa, subproducto de la elaboración de cerveza, contiene numerosos compuestos bioactivos. Una estrategia novedosa identificó análogos de ácidos amargos del lúpulo y potencial antioxidante, apoyando aplicaciones de valorización de residuos.
Área de la Ciencia:
- Biotecnología
- Química de Productos Naturales
- Química Analítica
Sus antecedentes:
- La tripa, un flujo de residuos de la elaboración de cerveza, es rica en metabolitos pero poco explorada.
- Los compuestos bioactivos de la tripa ofrecen potencial para aplicaciones nutracéuticas y farmacéuticas.
- Se necesitan métodos eficientes para caracterizar corrientes complejas de residuos biológicos.
Objetivo del estudio:
- Desarrollar y aplicar una estrategia multi-paso para la bioprospección de la tripa.
- Identificar y caracterizar metabolitos bioactivos dentro de la tripa.
- Evaluar el potencial de la tripa para la valorización sostenible de residuos.
Principales métodos:
- Extracción exhaustiva y análisis de cromatografía líquida de alta resolución/espectrometría de masas/espectrometría de masas (LC-HRMS/MS).
- Redes moleculares basadas en características (FBMN) para la desreplicación de metabolitos.
- Análisis multivariante no supervisado y perfilado de bioactividad (ensayos antioxidantes).
Principales resultados:
- Identificación de aproximadamente 700 metabolitos agrupados en extracto de etanol.
- Análisis putativo de 18 candidatos a análogos de ácidos α- y β-, ampliando la diversidad conocida de ácidos amargos del lúpulo.
- Potencial antioxidante observado vinculado a grupos de metabolitos específicos a través de análisis multivariante.
Conclusiones:
- La estrategia integrada de múltiples pasos permite una evaluación rápida y amplia de la diversidad química en las corrientes de residuos.
- La tripa es una fuente valiosa de compuestos bioactivos novedosos, incluidos análogos de ácidos amargos del lúpulo.
- Este enfoque apoya la valorización sostenible de residuos para las industrias nutracéutica y farmacéutica.
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