Video Experimental Relacionado

Updated: Jan 24, 2026

Establishment of Orthotopic Patient-derived Xenograft Models for Brain Tumors using a Stereotaxic Device

Establishment of Orthotopic Patient-derived Xenograft Models for Brain Tumors using a Stereotaxic Device

Published on: May 2, 2025

Protocolo para el ensayo Seahorse 3D Mito Stress en neuroesferas de xenoinjerto de tumores cerebrales pediátricos

Stefania Tocci, Jessica W Tsai

bioRxiv : the preprint server for biology

|January 23, 2026

Abstract:

Metabolism is essential for cellular functions and is often altered in cancer. While Seahorse assays are well established for measuring metabolic changes in 2D cell cultures, their application to 3D models remains challenging. Here, we present a step-by-step protocol for plating individual brain tumor neurospheres in the assay microplate to measure their bioenergetics. We provide actionable recommendations and highlight pitfalls to avoid. This approach enables exploration of dynamic metabolic changes in patient-derived brain tumor neurospheres following genetic or pharmacologic interventions.

Highlights:

Optimization of Seahorse assay protocol for single patient-derived brain tumor spheroidsStep-by-step instructions for successfully re-plating and transferring single brain tumor spheroidsImportance of microplate coating for the successful execution of Seahorse assaysImaging of the microplate before and after the assay allows for normalization of metabolic parameters based on neurosphere size.

Palabras clave:

metabolismo del cáncer ensayo Seahorse neuroesferas modelos 3D tumores cerebrales

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