Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Proteomics01:33

Proteomics

9.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.5K
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

9.8K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
9.8K
ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

6.2K
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
6.2K
Xylem and Transpiration-driven Transport of Resources02:03

Xylem and Transpiration-driven Transport of Resources

26.5K
The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
26.5K
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

4.7K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
4.7K
Cancer02:18

Cancer

53.9K
Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.
53.9K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Immunoelectroanalytical multiplexing of RNA methylation signatures to decode oncogenic point mutations in cancer cells.

Talanta·2026
Same author

Expression of Apolipoprotein L1 Risk Variants at the Plasma Membrane and Haplotype-Dependent Cytotoxicity.

Journal of the American Society of Nephrology : JASN·2026
Same author

Protocol for synthesis and mechanical characterization of polyacrylamide hydrogels of varying stiffness for cell culture applications.

STAR protocols·2026
Same author

Cannabinoid CB<sub>2</sub> receptor drives trastuzumab resistance and predicts durable anti-HER2 response.

Oncogene·2026
Same author

Label-free paper-based electrochemical aptasensor with tunable selectivity for assessing neurotransmitter imbalance in Alzheimer's disease.

Mikrochimica acta·2026
Same author

Localized heat induces ERK activation and signal propagation in solid tumors.

Scientific reports·2026

Video Experimental Relacionado

Updated: Jan 27, 2026

Orthotopic Mouse Model of Colorectal Cancer
08:43

Orthotopic Mouse Model of Colorectal Cancer

Published on: December 4, 2007

47.1K

Descifrando los cambios impulsados por la rigidez en el cáncer colorrectal mediante proteómica

Charlotte Cresens1, Ana Montero-Calle2, Guillermo Solís-Fernández3

  • 1Molecular Imaging and Photonics Division, Chemistry Department, Faculty of Sciences, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium.

Molecular & cellular proteomics : MCP
|January 25, 2026
PubMed
Resumen

El endurecimiento tumoral altera significativamente los secretomas de las células de cáncer colorrectal, mejorando la migración y la angiogénesis. Esto resalta la rigidez de la matriz

Palabras clave:
Rigidez tumoralcáncer colorrectalensayos funcionalesproteoma intracelular y secretadoproteómicasecretoma

Más Videos Relacionados

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
06:52

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

6.9K
A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
06:01

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Published on: July 6, 2017

9.9K

Videos de Experimentos Relacionados

Last Updated: Jan 27, 2026

Orthotopic Mouse Model of Colorectal Cancer
08:43

Orthotopic Mouse Model of Colorectal Cancer

Published on: December 4, 2007

47.1K
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
06:52

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

6.9K
A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
06:01

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Published on: July 6, 2017

9.9K

Área de la Ciencia:

  • Ingeniería Biomédica
  • Biología del Cáncer
  • Proteómica

Sus antecedentes:

  • El endurecimiento tumoral es un factor crítico en la progresión y metástasis del cáncer.
  • Las propiedades mecánicas del microambiente tumoral influyen en el comportamiento de las células cancerosas.
  • Existe una investigación limitada sobre el impacto de la rigidez tumoral en la expresión de proteínas.

Objetivo del estudio:

  • Investigar el efecto de la rigidez de la matriz en la desregulación de proteínas en el cáncer colorrectal.
  • Identificar cambios proteicos específicos en respuesta a la alteración de la rigidez de la matriz.
  • Comprender cómo estos cambios proteicos influyen en la progresión del cáncer.

Principales métodos:

  • Análisis proteómico en profundidad de células de cáncer colorrectal.
  • Comparación de la expresión de proteínas en diversas condiciones de rigidez de la matriz.
  • Ensayos funcionales para evaluar la migración celular, la angiogénesis y la remodelación de la matriz.

Principales resultados:

  • La rigidez de la matriz alteró significativamente la expresión de proteínas secretadas (secretoma).
  • Los niveles de proteínas intracelulares permanecieron en gran medida sin afectar por los cambios en la rigidez de la matriz.
  • Los cambios en el secretoma inducidos por la rigidez promovieron la migración celular, la angiogénesis y la remodelación de la matriz.

Conclusiones:

  • La rigidez de la matriz juega un papel crítico en la progresión del cáncer colorrectal a través de alteraciones del secretoma.
  • Los cambios en el secretoma contribuyen a un fenotipo de cáncer más agresivo.
  • Los hallazgos ofrecen información para el desarrollo de nuevas terapias contra el cáncer biomecánicas.