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Interneuronas Shox2 de la médula espinal lumbar reciben entrada excitatoria monosináptica del núcleo
Shayna Singh1, Lihua Yao1, Kimberly J Dougherty1
1Marion Murray Spinal Cord Research Center, Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USA.
iScience
|January 26, 2026
Resumen
La iniciación de la locomoción involucra vías del tronco encefálico. Los investigadores encontraron conexiones excitatorias directas desde el núcleo paragigantocellular lateral (LPGi) a las interneuronas Shox2 en la médula espinal, cruciales para la locomoción de los vertebrados.
Área de la Ciencia:
- Neurociencia
- Control Motor
- Investigación de la Médula Espinal
Sus antecedentes:
- La locomoción de los vertebrados es controlada por redes de la médula espinal, iniciada por centros supraspinales.
- Las interneuronas espinales que expresan Shox2 (interneuronas Shox2) están implicadas en la generación de ritmos locomotores.
- Las neuronas reticuloespinales, particularmente las neuronas excitatorias en el núcleo paragigantocellular lateral (LPGi), proporcionan el impulso descendente para la iniciación de la locomoción.
Objetivo del estudio:
- Investigar las conexiones sinápticas directas entre las neuronas excitatorias del LPGi y las interneuronas Shox2 lumbares en ratones adultos.
- Elucidar el papel de la vía LPGi-Shox2 en la iniciación de la locomoción de los vertebrados.
Principales métodos:
- Se utilizó el rastreo viral de rabia restringido a sinapsis únicas para el rastreo preciso de conexiones neuronales.
- Se emplearon técnicas de rastreo anterógrado para confirmar la direccionalidad de la vía.
- Se realizaron registros electrofisiológicos para medir los potenciales postsinápticos excitatorios (EPSPs) en las interneuronas Shox2.
Principales resultados:
- Se demostraron conexiones sinápticas directas de neuronas excitatorias en el LPGi a interneuronas Shox2 lumbares.
- Se confirmó esta entrada excitatoria desde la médula caudal ventral a las interneuronas Shox2 utilizando múltiples métodos de rastreo.
- La electrofisiología verificó potenciales postsinápticos excitatorios funcionales en las interneuronas Shox2 después de la estimulación del LPGi.
Conclusiones:
- Un subconjunto de interneuronas Shox2 recibe entrada excitatoria monosináptica directa del LPGi.
- Esta vía neural identificada proporciona un sustrato potencial para la iniciación de la locomoción.
- Destaca la importancia del LPGi en la coordinación del control supraspinal de los circuitos motores espinales.
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