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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
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Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Fungi are a diverse group of eukaryotes more closely related to animals than other eukaryotes. Fungal cell walls comprise chitin, a polysaccharide that provides structural strength, and glucans, which contribute to flexibility and integrity. Other polysaccharides, such as mannans and galactosans, may supplement or replace chitin in some fungi. These adaptations, along with their preference for acidic environments and tolerance for high osmotic pressure, enable fungi to thrive in various...
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Updated: Jan 28, 2026

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Genomas T2T de Colletotrichum lini con Hifiasm: Directrices de procesamiento y ensamblaje de lecturas ONT R9 y R10

Elizaveta A Ivankina1, Ekaterina M Dvorianinova1, Alexander A Arkhipov1,2

  • 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.

Journal of fungi (Basel, Switzerland)
|January 27, 2026
PubMed
Resumen

Los parámetros optimizados de secuenciación Oxford Nanopore Technologies (ONT) y ensamblaje Hifiasm permiten ensamblajes completos del genoma de telómero a telómero (T2T) para patógenos fúngicos como Colletotrichum lini. Esto facilita los estudios genómicos y la comprensión de la enfermedad de la antracnosis del lino.

Sus antecedentes:

  • El ensamblaje del genoma de telómero a telómero (T2T) es crucial para comprender los genomas complejos de patógenos fúngicos.
Palabras clave:
Colletotrichum liniNanoporeceldas de flujo R9.4.1 y R10.4.1ensamblaje del genoma T2Tantracnosisherramientas de basecallingpatógeno del linoprocesamiento de lecturas

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  • Colletotrichum lini causa una enfermedad significativa de antracnosis del lino, lo que requiere un análisis genómico detallado.

    • Conclusiones:

    • El preprocesamiento optimizado de datos ONT y el ensamblaje Hifiasm producen genomas T2T de alta calidad para C. lini.
    • Se estableció un marco robusto para el ensamblaje del genoma fúngico utilizando la tecnología ONT.
    • Los hallazgos respaldan la investigación futura sobre la variación genómica y la patogenicidad en especies de Colletotrichum.