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Microfluídica Esculpida Magnéticamente para Fraccionamiento Continuo de Poblaciones Celulares por Nivel de Expresión
Zhenwei Liang1, Xiaolei Guo1,2, Xuanhe Zhang1
1Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
Micromachines
|January 28, 2026
Resumen
Este estudio presenta una plataforma microfluídica que utiliza tiras magnéticas blandas para la separación continua de células basada en los niveles de marcadores de superficie. Permite el fraccionamiento celular cuantitativo guiado por marcadores con alta recuperación y viabilidad para aplicaciones posteriores.
Área de la Ciencia:
- Ingeniería Biomédica
- Microfluídica
- Tecnología de Separación Celular
Sus antecedentes:
- La separación de flujo continuo de células marcadas magnéticamente es crucial para estudiar la heterogeneidad celular y apoyar ensayos.
- Los métodos existentes requieren un control preciso de los campos magnéticos y el marcado celular para un fraccionamiento eficaz.
Objetivo del estudio:
- Desarrollar una plataforma microfluídica para la separación celular cuantitativa de flujo continuo basada en los niveles de expresión de marcadores de superficie.
- Establecer una estrategia de diseño de campo magnético para el fraccionamiento celular dependiente del nivel de expresión utilizando elementos magnéticos blandos.
Principales métodos:
- Se utilizaron tiras magnéticas blandas (SMS) diseñadas espacialmente para crear campos de deflexión magnética lateral en un microcanal.
- Se empleó un marco COMSOL-MATLAB y una métrica de fuerza equivalente para optimizar los parámetros estructurales de SMS.
- Se implementó una cascada de tres etapas con recolección de múltiples salidas para la partición celular en subgrupos discretos de nivel de expresión.
Principales resultados:
- Se particionaron con éxito células MDA-MB-231, Caco-2 y A549 en cuatro subgrupos magnéticos relacionados con EpCAM (alto, medio, bajo, casi negativo).
- Se logró una alta recuperación celular (>90%) y se mantuvo la viabilidad celular (98.2 ± 1.3%).
- Se demostró que las fracciones separadas se alinean con las tendencias de expresión conocidas en la literatura.
Conclusiones:
- La plataforma microfluídica desarrollada permite el fraccionamiento celular cuantitativo de flujo continuo basado en la expresión de marcadores de superficie.
- La estrategia de diseño de campo magnético utilizando SMS es eficaz para los sistemas de separación magneto-fluídicos de próxima generación.
- El sistema es compatible con aplicaciones posteriores, incluidos ensayos de sangre completa y cultivo celular.
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