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Updated: Jan 30, 2026

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

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Fotoactivación de la imagen de localización por ultrasonido con nanogotas activadas por láser

Shensheng Zhao^1,2,3, Junxi Yi^2,3,4, Yueying Qiu^1,2,3

¹Department of Electrical and Computer Engineering, Grainger College of Engineering, University of Illinois Urbana-Champaign, Urbana, IL, USA.

Communications engineering

|January 28, 2026

Resumen

La imagen de localización activada por foto (PaUL) utiliza nanogotas activadas por láser para la visualización microvascular de super-resolución. Este novedoso método ofrece un control preciso, imágenes más rápidas y un seguimiento extendido para un mapeo hemodinámico mejorado.

Área de la Ciencia:

Imagen Biomédica
Nanotecnología
Tecnología de Ultrasonido

Sus antecedentes:

La imagen de localización ultrasónica de super-resolución (UL) visualiza la microvasculatura más allá del límite de difracción.
La UL convencional está limitada por el tiempo de circulación y la distribución de las microburbujas.

Objetivo del estudio:

Introducir la imagen UL activada por foto (PaUL) para la visualización microvascular de super-resolución.
Lograr un control espaciotemporal preciso sobre la generación de contraste utilizando nanogotas activadas por láser.

Principales métodos:

Síntesis de nanogotas de perfluoropentano encapsuladas con verde de indocianina (ICG) de doble contraste.
Desarrollo de una secuencia programable de activación e imagen para PaUL.
Utilización de la fluencia del láser para ajustar la generación de contraste bajo demanda.

Principales resultados:

La imagen PaUL logró una resolución espacial de 21 µm.
La reconstrucción vascular fue 2.4 veces más rápida y las longitudes de seguimiento fueron 1.6 veces más largas en comparación con la UL convencional.
El tiempo de circulación extendido permitió hasta 3 veces más eventos de localización en 20 minutos.

Conclusiones:

La imagen PaUL proporciona contraste flexible de alta resolución con control espaciotemporal preciso.
La integración con la imagen fotoacústica mejora las capacidades multimodales.
La imagen PaUL muestra una gran promesa para la imagen microvascular y las terapias guiadas por imagen, como la administración dirigida de fármacos.

Palabras clave:

nanogotas ultrasonido imagen de super-resolución microvasculatura láser imagen fotoacústica nanotecnología

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