Video Experimental Relacionado

Updated: Jan 31, 2026

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

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Comprensión de la separación de fases líquido-líquido a través de TDP-43: principios fundamentales, compartimentación

Alessandra Bigi¹, Fabrizio Chiti²

¹Department of Experimental and Clinical Biomedical Sciences, Section of Biochemistry, University of Florence, Florence, 50134, Italy. alessandra.bigi@unifi.it.

|January 30, 2026

Abstract:

Phase separation is an important process in biology associated with formation of membraneless organelles but possibly related to the emergence of solid inclusions. TDP-43 is a largely studied paradigmatic case, as it forms neuronal cytoplasmic inclusions in neurodegenerative diseases and is an essential component of many membraneless organelles. Here, we review the physicochemical fundamentals of liquid-liquid phase separation (LLPS) of TDP-43 and its fragments in vitro, showing that full-length TDP-43 requires RNA or chaperones to form stable liquid droplets. We describe TDP-43-containing membraneless organelles and the debate on whether these assemblies represent reservoirs for pathological solid inclusion formation.

Palabras clave:

Esclerosis lateral amiotrófica Anisóforos FTLD-TDP LATE-NC Paraspeckles Gránulos de estrés Proteína de unión a ADN TAR 43

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