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RNA Stability01:53

RNA Stability

35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Classification of Skeletal Muscle Fibers01:48

Classification of Skeletal Muscle Fibers

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Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
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Muscles of the Eye01:20

Muscles of the Eye

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The muscles of the eye are sophisticated structures that control eye movement and focus, allowing for the precise and rapid adjustments necessary for vision. The human eye is controlled by ten muscles — six extraocular muscles, three intraocular muscles, and one primary eyelid retractor muscle.
Extraocular Muscles
The six extraocular muscles surround the eyeball and control its movements. They are responsible for a wide range of eye motions, including looking up, down, left, right, and...
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Muscles that Move the Head01:19

Muscles that Move the Head

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The muscles that move the head are a dynamic and complex group of structures that work together to facilitate a wide range of head movements, including rotation, flexion, extension, and lateral bending.
The bilateral sternocleidomastoid, or SCM, and the suprahyoid and infrahyoid muscles are significant head flexors. The SCM muscles originate at the sternum and clavicle and attach to the mastoid process of the temporal bone. The SCM contracts bilaterally to bend the head forward, whereas...
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Muscles of the Abdomen01:21

Muscles of the Abdomen

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The abdominal wall encircles the abdominal cavity, providing flexible protection and shielding the internal organs from harm. It is bordered at the top by the xiphoid process and costal margins, at the back by the vertebral column, and at the bottom by the pelvic bones and inguinal ligament. The abdominal wall is divided into two regions — the anterolateral and posterior regions.
Anterolateral Region
The anterolateral region comprises five paired muscles classified into the lateral and...
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Muscles that Move the Arm01:31

Muscles that Move the Arm

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Nine muscles are involved in arm movements. Two of these, the pectoralis major and latissimus dorsi, originate from the axial skeleton and are called axial muscles. The other seven originate from the scapula and are called the scapular muscles.
The pectoralis major has two origins. Its clavicular head originates on the medial half of the clavicle. In contrast, the sternocostal head originates on the costal cartilages of ribs 1-6, the sternum, and the aponeurosis of the external oblique of the...
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Video Experimental Relacionado

Updated: Jan 31, 2026

Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice
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Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice

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ARN circular circSnd1 inducido por EIF4A3 promueve la atrofia muscular y el envejecimiento muscular al estabilizar

Jin Li1,2, Bing Jin1,2, Yuwei Yan1,2

  • 1Cardiac Regeneration and Ageing Lab, Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong) and School of Life Sciences, Shanghai University, Nantong, China.

Journal of cachexia, sarcopenia and muscle
|January 30, 2026
PubMed
Resumen

El ARN circular circSnd1 promueve la atrofia y el envejecimiento muscular al estabilizar la proteína EEF1A1. La inhibición de circSnd1 mejora el desgaste muscular, ofreciendo una diana terapéutica potencial para la atrofia muscular.

Palabras clave:
ubiquitinación de EEF1A1EIF4A3FAT10circSnd1envejecimiento muscularatrofia muscular

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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Área de la Ciencia:

  • Biología Molecular
  • Biología Celular
  • Gerontología

Sus antecedentes:

  • La atrofia muscular es una complicación importante del envejecimiento y de las enfermedades crónicas.
  • Las intervenciones terapéuticas actuales para la atrofia muscular son limitadas.
  • El papel de los ARN circulares (ARNc) en la atrofia muscular sigue siendo en gran medida inexplorado.

Objetivo del estudio:

  • Investigar la función y el mecanismo de circSnd1 en la atrofia y el envejecimiento muscular.
  • Identificar los ARN circulares implicados en el desgaste muscular.

Principales métodos:

  • Secuenciación de ARN circular y PCR cuantitativa de transcripción inversa en tiempo real en modelos de atrofia muscular y músculo envejecido.
  • Experimentos de ganancia y pérdida de función para evaluar el papel de circSnd1.
  • Inmunoprecipitación de ARN seguida de espectrometría de masas (RIP-MS) y ensayo RIP para dilucidar los mecanismos moleculares.

Principales resultados:

  • circSnd1, un ARN circular conservado del gen SND1, se sobreexpresa en modelos de atrofia muscular y en músculo de ratón y humano envejecido.
  • circSnd1 promueve la atrofia y el envejecimiento muscular en modelos celulares y animales.
  • La expresión de circSnd1 está regulada por EIF4A3 y promueve la atrofia muscular al estabilizar la proteína EEF1A1 a través de la interacción con FAT10, reduciendo la ubiquitinación de EEF1A1.

Conclusiones:

  • Se identifica circSnd1 como un ARN circular novedoso que promueve la atrofia y el envejecimiento muscular.
  • circSnd1 representa una diana terapéutica potencial para combatir las afecciones de desgaste muscular.