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Las células cultivadas activan IRE1 durante la adhesión y el aplanamiento tras el pase rutinario
Paige Dillon1, Lincoln Hollingshead1, Julie Hollien1
1School of Biological Sciences, University of Utah, Salt Lake City, Utah, United States.
microPublication biology
|January 30, 2026
Resumen
El pase celular rutinario desencadena el empalme de Xbp1, activando la respuesta a proteínas desplegadas. Esto ocurre independientemente del estrés del ER, lo que sugiere que las células inician esta vía durante la readhesión y la extensión después de la subcultivación.
Área de la Ciencia:
- Biología celular
- Biología molecular
- Bioquímica
Sus antecedentes:
- El estrés del retículo endoplásmico (ER) típicamente activa la respuesta a proteínas desplegadas (UPR) a través del empalme del ARNm de Xbp1 mediado por IRE1.
- Este proceso genera un factor de transcripción crucial para restaurar la proteostasis.
Objetivo del estudio:
- Investigar si el empalme de Xbp1 ocurre durante los procedimientos de cultivo celular rutinarios sin estresores artificiales del ER.
- Determinar los factores que influyen en el empalme de Xbp1 durante el pase celular.
Principales métodos:
- Cultivo de células de ratón MC3T3-E1.
- Realización de pases celulares rutinarios utilizando diversos tampones de disociación.
- Evaluación del empalme de Xbp1.
- Utilización de placas de cultivo no adherentes.
Principales resultados:
- Se observó el empalme de Xbp1 en células cultivadas durante el pase rutinario, independientemente del estrés del ER.
- El tipo de tampón de disociación no afectó al empalme de Xbp1.
- Se redujo el empalme cuando las células se cultivaron en superficies no adherentes.
Conclusiones:
- Las células de mamíferos cultivadas pueden iniciar la respuesta a proteínas desplegadas durante los procesos mecánicos y de adhesión de la readhesión y la extensión posteriores al pase.
- El microambiente celular y las señales físicas durante el pase pueden influir en la activación de la UPR.
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