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Un AIEgen activable por Caspasa-3 en el infrarrojo cercano para la obtención de imágenes de apoptosis tumoral in vivo

Lingling Xu^1,2, Yuanyuan Jin², Zhanjun Yang²

¹Breast Surgery, Wenzhou Central Hospital, Wenzhou 325000, China.

Chemical & biomedical imaging

|January 30, 2026

Resumen

Los investigadores desarrollaron un nuevo luminógeno de agregación-inducción de emisión (AIEgen) en el infrarrojo cercano (NIR) para rastrear la eficacia de los fármacos contra el cáncer mediante la obtención de imágenes de la actividad de la caspasa-3 en tumores. Este nuevo AIEgen permite la monitorización sensible y en tiempo real de las respuestas terapéuticas in vivo.

Área de la Ciencia:

Imagenología Biomédica
Nanotecnología
Biología Química

Sus antecedentes:

La obtención de imágenes por fluorescencia en el infrarrojo cercano (NIR) permite la monitorización en tiempo real de la eficacia de los fármacos contra el cáncer mediante la detección de la actividad de la caspasa-3 tumoral.
Los luminógenos de agregación-inducción de emisión (AIEgens) son fluoróforos sensibles, pero faltan AIEgens NIR para la obtención de imágenes de la actividad de la caspasa-3.

Objetivo del estudio:

Desarrollar un AIEgen NIR activable para la obtención de imágenes sensible de la actividad de la caspasa-3 en células y tumores apoptóticos.
Evaluar el potencial del AIEgen desarrollado para la monitorización en tiempo real de los efectos terapéuticos de los fármacos contra el cáncer.

Principales métodos:

Síntesis de un AIEgen NIR activable, Ac-Asp-Glu-Val-Asp-Pra-QMT (Ac-DEVD-Pra-QMT).
Experimentos in vitro e in vivo para evaluar la obtención de imágenes de la actividad de la caspasa-3 en células y tumores apoptóticos inducidos por cisplatino.
Medición de los cambios en la intensidad de la fluorescencia NIR tras la escisión por caspasa-3 y la agregación.

Principales resultados:

Se desarrolló con éxito Ac-DEVD-Pra-QMT como un AIEgen NIR activable.
Tras la escisión por la caspasa-3 en células apoptóticas, Ac-DEVD-Pra-QMT forma nanopartículas, activando la fluorescencia NIR.
La intensidad de la fluorescencia NIR mostró un aumento de 14,9 veces in vitro y un aumento de 2,7 veces in vivo en comparación con los controles.

Conclusiones:

Ac-DEVD-Pra-QMT obtiene imágenes de manera efectiva de la actividad de la caspasa-3 en células y tumores apoptóticos.
El AIEgen NIR desarrollado muestra un potencial significativo para el seguimiento sensible y en tiempo real de los efectos quimioterapéuticos.
Esta herramienta podría ayudar en la monitorización temprana de las respuestas al tratamiento en la terapia contra el cáncer.

Palabras clave:

AIEgens caspasa-3 imagen in vivo infrarrojo cercano apoptosis tumoral

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