Métodos de alto rendimiento que aprovechan la robótica y la visión por computadora para el desarrollo de cócteles de...

Área de la Ciencia:

• Microbiology
• Biotechnology
• Infectious Diseases

Sus antecedentes:

• Bacteriophage therapy is a promising alternative to antibiotics for bacterial infections.
• Urinary tract infections (UTIs) are a growing concern due to rising antibiotic resistance.
• Developing scalable and reproducible methods for bacteriophage cocktail development is crucial.

Objetivo del estudio:

• To present high-throughput automated screening techniques for bacteriophage therapeutic product development.
• To establish a platform for reproducible screening of large phage collections against bacterial pathogens.
• To facilitate the identification and optimization of phage cocktails for combating antibiotic resistance.

Principales métodos:

• Integration of liquid handling robotics, standardized phenotypic assays, and computer vision-based enumeration.
• Systematic assessment of phage-bacteria interactions at scale using clinically derived bacterial strains.
• Development of a platform for reproducible and scalable bacteriophage cocktail development.

Principales resultados:

• Established a platform capable of reproducibly screening large phage collections.
• Enabled systematic assessment of phage-bacteria interactions at scale.
• Facilitated the identification and optimization of phage cocktails with broad in vitro activity.

Conclusiones:

• The presented methods address the need for scalable and reproducible bacteriophage cocktail development.
• This platform supports the creation of novel therapeutic assets to combat antibiotic resistance.
• Automated screening techniques are vital for advancing bacteriophage-based therapies in clinical trials.