Video Experimental Relacionado

Updated: Feb 1, 2026

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

ARN circular Vv-circCOR27 modula la termotolerancia al atenuar la interacción VvHSP90.2b-VvHsfA7a en la vid

Yi Ren^1,2, Yuanyuan Xu², Moyang Liu²

¹College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.

The New phytologist

|January 31, 2026

Resumen

Los ARN circulares (circRNAs) regulan la termotolerancia de la vid uniéndose a la proteína de choque térmico 90.2b (VvHSP90.2b), inhibiendo las interacciones que controlan los genes de respuesta al estrés térmico.

Área de la Ciencia:

Biología molecular vegetal
Biología de ARN
Fisiología del estrés

Sus antecedentes:

Los ARN circulares (circRNAs) son moléculas reguladoras clave en las plantas, pero sus funciones siguen sin explorarse en gran medida.
Comprender los mecanismos reguladores de los circRNAs es crucial para mejorar la tolerancia de las plantas al estrés.

Objetivo del estudio:

Investigar la función y los mecanismos reguladores del ARN circular de vid Vv-circCOR27.
Elucidar el papel de Vv-circCOR27 en la termotolerancia de las plantas.

Principales métodos:

Transformación genética, ensayos de captura de ARN circular e inmunoprecipitación de ribonucleoproteínas (RIP).
Se utilizó un sistema de complementación de fluorescencia trimolecular de ARN circular (cTriFC).
Sobreexpresión de Vv-circCOR27 utilizando un casete de expresión especializado.

Principales resultados:

La expresión de Vv-circCOR27 disminuyó bajo estrés térmico y fue menor en cultivares de vid termotolerantes.
La sobreexpresión de Vv-circCOR27 mejoró la termotolerancia en la vid.
Vv-circCOR27 se une directamente a VvHSP90.2b, inhibiendo la interacción VvHsfA7a y la regulación al alza de los genes de pequeñas proteínas de choque térmico (sHSP).

Conclusiones:

Vv-circCOR27 ajusta finamente la termotolerancia al unirse competitivamente a VvHSP90.2b.
Esta interacción atenúa la unión de VvHsfA7a y la posterior transactivación de los genes de respuesta al estrés térmico.
El estudio avanza la comprensión de los mecanismos reguladores de los circRNAs en la respuesta de las plantas al estrés térmico.

Palabras clave:

HSP90 ARN circular vid factor de choque térmico estrés térmico

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