Video Experimental Relacionado

Updated: Feb 1, 2026

Murine Model of Wound Healing

Murine Model of Wound Healing

Published on: May 28, 2013

Bacteriófago y óxido nítrico liberados combinadamente de una matriz de hidrogel dual para aplicaciones de

Sarah N Wilson¹, Adam B Goodman¹, Aasma Sapkota¹

¹School of Chemical, Materials, and Biological Engineering, College of Engineering, University of Georgia, Athens, Georgia, USA.

Macromolecular bioscience

|January 31, 2026

Abstract:

Infection sites and open wounds provide a prime environment for the growth of opportunistic pathogens, leading to persistent infections caused by bacteria that contain antimicrobial-resistant phenotypes. Mistreatment of these wound infections often increases antimicrobial resistance (AMR), thereby decreasing the effectiveness of antibiotics. With the increase in AMR, new antimicrobial therapeutics that target these hard-to-kill pathogens are needed. Herein, naturally harvested bacteriophages (ECΦ) were combined with another established antimicrobial molecule, nitric oxide (NO). This combination has rarely been explored in biomedical devices but shows excellent potential for developing broad-spectrum, antibacterial materials. Bacteriophages were encapsulated in alginate microbeads and suspended in a NO-releasing thermoresponsive hydrogel. The phages were shown to have a delayed release from the alginate beads when incorporated into the gel, compared to the release observed within 24 h in aqueous medium. This delayed release enabled tunable phage delivery by adjusting the viscosity of the bulk gel base. Additionally, we used alginate as the base for microbeads, resulting in a physiologically safe material due to its proven biocompatibility. The final ECΦ and NO-releasing bead-gel matrix demonstrated 5-10 times larger zones of bacterial killing while maintaining low cytotoxicity, enabling further development in various clinical applications, including wound healing.

Palabras clave:

bacteriófagos hidrogel microesferas óxido nítrico

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