Video Experimental Relacionado

Updated: Feb 3, 2026

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Published on: December 20, 2019

Estudio funcional de la alanina aminotransferasa: la sintetasa del compuesto umami clave, la alanina, en Urechis

Yanqing Ma¹, Kai Wang¹, Tianya Yang¹

¹Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.

International journal of biological macromolecules

|February 1, 2026

Abstract:

Umami, the fifth basic taste alongside sour, sweet, bitter, and salty, is primarily influenced by free amino acids (FAAs), organic acids and inorganic ions, as indicated by Taste Activity Value (TAV). Urechis unicinctus, valued for its distinctive flavor and nutritional value, was selected to explore the molecular basis of its umami taste. Unlike most other seafood, its umami flavor originated from high concentration of alanine (Ala) in FAAs. The results showed that variations in Ala content between different cultured populations may be associated with glutamic-pyruvic transaminase (GPT) activity and gene expression levels. Functional studies confirmed that gpt interference led to a significant decrease in Ala and key umami-related amino acids in muscle tissue. Furthermore, mutagenesis analysis revealed that the conserved and evolutionary adaptive residues (K298, S297, M294, N239) are critical for GPT catalytic activity. Taken together, our findings provide new insights into the mechanisms underlying umami formation in seafood.

Palabras clave:

GPT Mutagénesis dirigida a sitio Valor de actividad gustativa Urechis unicinctus

Más Videos Relacionados

Laparoscopic Anatomic S7+S8d Resection Preserving Inferior Right Hepatic Vein and S6 with Right Hepatic Vein Transection

Laparoscopic Anatomic S7+S8d Resection Preserving Inferior Right Hepatic Vein and S6 with Right Hepatic Vein Transection

Published on: December 30, 2025

A Purification and In Vitro Activity Assay for a pppGpp Synthetase from Clostridium difficile

A Purification and In Vitro Activity Assay for a pppGpp Synthetase from Clostridium difficile

Published on: November 3, 2018

Videos de Conceptos Relacionados

Key Elements for Plant Nutrition

Key Elements for Plant Nutrition

Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...

Compounds Essential to Human Function

Compounds Essential to Human Function

The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all physiological processes in the body and are broadly classified into organic and inorganic based on their chemical structures.
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...

Organic Compounds

Organic Compounds

All living things are formed mostly of carbon compounds called organic compounds. The category of organic compounds includes both natural and synthetic compounds that contain carbon. Although a single, precise definition has yet to be identified by the chemistry community, most agree that a defining trait of organic molecules is the presence of carbon as the principal element, bonded to hydrogen and other carbon atoms. However, some carbon-containing compounds such as carbonates, cyanides, and...

Key Techniques in Microbiology

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...

Molecules and Compounds

Molecules and Compounds

Atoms and Molecules

Coordination Compounds and Nomenclature

Coordination Compounds and Nomenclature

In most main group element compounds, the valence electrons of the isolated atoms combine to form chemical bonds that satisfy the octet rule. For instance, the four valence electrons of carbon overlap with electrons from four hydrogen atoms to form CH4. The one valence electron leaves sodium and adds to the seven valence electrons of chlorine to form the ionic formula unit NaCl (Figure 1a). Transition metals do not normally bond in this fashion. They primarily form coordinate covalent bonds, a...