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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Classifying Matter by Composition03:35

Classifying Matter by Composition

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Matter: Pure Substances and Mixtures
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures. 
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated. 
A mixture is composed of two or...
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Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

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Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
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Video Experimental Relacionado

Updated: Feb 4, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

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Ingeniería de la Composición de Ésteres de Cera en Euglena gracilis Mediante Edición Genómica

Sakura Nagamine1, Rikuto Oishi1, Masami Nakazawa2

  • 1Department of Applied Biochemistry, Faculty of Agriculture, Osaka Metropolitan University, Sakai, Osaka, Japan.

Methods in molecular biology (Clifton, N.J.)
|February 2, 2026
PubMed
Resumen
Este resumen es generado por máquina.

Este estudio detalla un protocolo de edición genómica CRISPR/Cas9 para Euglena gracilis para modificar la producción de ésteres de cera. Este método permite la ingeniería genética estable de E. gracilis para aplicaciones de biología sintética y biofabricación.

Palabras clave:
Metabolismo anaeróbicoCRISPR/Cas9Euglena gracilisβ-oxidación de ácidos grasosEdición genómicaPérdida de funciónModificación metabólicaKnockout génico dirigidoSíntesis de ésteres de cera

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Last Updated: Feb 4, 2026

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Área de la Ciencia:

  • Biología Sintética
  • Ingeniería Metabólica
  • Genómica

Sus antecedentes:

  • Los avances en la edición genómica permiten la ingeniería metabólica en organismos no modelo como Euglena gracilis.
  • Los sistemas CRISPR/Cas9 y CRISPR/Cas12a se han establecido para la edición genómica de E. gracilis.

Objetivo del estudio:

  • Proporcionar un protocolo detallado para la edición genómica basada en CRISPR/Cas9 en E. gracilis.
  • Permitir la modificación estable de la composición de ésteres de cera en condiciones anaeróbicas.

Principales métodos:

  • Utilización de la tecnología CRISPR/Cas9 para dirigirse a enzimas clave en la vía de β-oxidación inversa.
  • Generación de mutantes knockout para alterar las longitudes de cadena de los ésteres de cera en E. gracilis.

Principales resultados:

  • Se generaron con éxito mutantes knockout de E. gracilis con longitudes de cadena de ésteres de cera alteradas.
  • Se estableció un método reproducible y estable para la modificación genética del metabolismo de E. gracilis.

Conclusiones:

  • El protocolo CRISPR/Cas9 desarrollado facilita la modificación genética estable del metabolismo de E. gracilis.
  • Este enfoque apoya el uso de E. gracilis como un chasis verde para la biología sintética y la biofabricación, con potencial para estrategias de knock-in.