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Interferencia del Dobesilato de Calcio en Dos Métodos de Detección de Proteínas Totales en Orina

Zheng Mingqi1,2, Ma Xiaoli1, Yu Songlin1

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Resumen

El dobesilato de calcio no interfiere con el método de piragallol rojo-molibdato para la detección de proteínas totales en orina. Sin embargo, interfiere significativamente con el método de cloruro de benzalconio, afectando los resultados según las concentraciones de fármaco y proteína.

Palabras clave:
cloruro de benzalconiodobesilato de calciointerferenciaproteína total en orinamuestras de orina

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Área de la Ciencia:

  • Química Clínica
  • Química Analítica
  • Farmacología

Sus antecedentes:

  • La medición de proteínas totales en orina es crucial para el diagnóstico y seguimiento de enfermedades renales.
  • El dobesilato de calcio es un medicamento que puede estar presente en las muestras de orina.
  • Es necesaria una evaluación cuidadosa de la posible interferencia de los medicamentos en los ensayos de diagnóstico.

Objetivo del estudio:

  • Investigar la interferencia del dobesilato de calcio en dos métodos comunes de detección de proteínas totales en orina.
  • Cuantificar la extensión y naturaleza de la interferencia causada por concentraciones variables de dobesilato de calcio.

Principales métodos:

  • Se recolectaron muestras de orina de 24 horas y de punto de pacientes con dobesilato de calcio.
  • Se cuantificaron las concentraciones del fármaco mediante cromatografía líquida-espectrometría de masas en tándem.
  • Se evaluó la interferencia mediante pruebas de diferencia pareada y experimentos de dosis-efecto.

Principales resultados:

  • El dobesilato de calcio no interfirió significativamente con el método de piragallol rojo-molibdato.
  • Se observó una interferencia significativa con el método de cloruro de benzalconio, mostrando un efecto en "forma de J".
  • La interferencia varió con las concentraciones de dobesilato de calcio y proteína en orina, oscilando entre -12.1% y +286.4% de desviación.

Conclusiones:

  • El método de piragallol rojo-molibdato es confiable para la detección de proteínas totales en orina en pacientes que toman dobesilato de calcio.
  • El método de cloruro de benzalconio es susceptible a la interferencia del dobesilato de calcio, lo que afecta la precisión.
  • Se recomienda suspender el dobesilato de calcio durante 1-2 días antes de la recolección de orina cuando se utiliza el método de cloruro de benzalconio.