Video Experimental Relacionado

Updated: Feb 5, 2026

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Published on: September 6, 2024

Electrófilos de Fósforo Insaturado para Investigar Fosfatasas de Tirosina de Proteínas

Eleftheria Poulou^1,2, Max Ruwolt¹, Christian E Stieger¹

¹Department of Chemical Biology, Leibniz-Forschungsinstitut Für Molekulare Pharmakologie (FMP), Berlin, Germany.

Angewandte Chemie (International ed. in English)

|February 4, 2026

Resumen

Los investigadores desarrollaron novedosas sondas que imitan la fosfotirosina para una focalización precisa de la fosfatasa de tirosina de proteína (PTP). Estas sondas marcan selectivamente las PTP, ayudando a comprender su papel en vías biológicas como la señalización de la insulina.

Sus antecedentes:

Las fosfatasas de tirosina de proteína (PTP) son objetivos farmacológicos cruciales.
Las sondas existentes carecen de alta selectividad de diana y accesibilidad sintética.
Los compuestos de fósforo (V) insaturado muestran promesa para la bioconjugación selectiva de cisteína.

Conclusiones:

Se estableció una estrategia versátil para crear sondas de PTP precisas basadas en péptidos.
Estas sondas facilitan la caracterización específica de las interacciones de las PTP.
Las sondas desarrolladas ofrecen una herramienta valiosa para estudiar las PTP en sistemas biológicos.

Palabras clave:

sondas basadas en actividad fosfatasa fósforo proteómica reactividad inducida por proximidad

Más Videos Relacionados

Preparation and Use of Carbonyl-decorated Carbenes in the Activation of White Phosphorus

Preparation and Use of Carbonyl-decorated Carbenes in the Activation of White Phosphorus

Published on: October 3, 2014

Laboratory-determined Phosphorus Flux from Lake Sediments as a Measure of Internal Phosphorus Loading

Laboratory-determined Phosphorus Flux from Lake Sediments as a Measure of Internal Phosphorus Loading

Published on: March 6, 2014

Videos de Conceptos Relacionados

Protein Kinases and Phosphatases

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...

Protein Kinases and Phosphatases

Protein Kinases and Phosphatases

Degree of Unsaturation

Degree of Unsaturation

The degree of unsaturation (U), or index of hydrogen deficiency (IHD), is defined as the difference in the number of pairs of hydrogen atoms between the compound and the acyclic alkane with the same number of carbon atoms. Each double bond or ring costs two hydrogen atoms compared to a saturated analog and results in one degree of unsaturation.
The degree of unsaturation for hydrocarbons is U = (2C + 2 − H) / 2, where C is the number of carbon atoms and H is the number of hydrogen atoms.

Electrophiles

Electrophiles

This lesson explains the definition, classification, and characteristic features of an electrophile that are key features of nucleophilic substitution reactions. An analysis of their charge and orbital picture helps understand their reactivity for seeking electrons. Electrophiles can be classified into positive and neutral species. Other classes include free radicals and polar functional groups.
While a positive electrophile, like a proton, reacts due to its vacant, low-energy 1s orbital, the...

Receptor Tyrosine Kinases

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...

The Phosphorus Cycle

The Phosphorus Cycle

Unlike carbon, water, and nitrogen, phosphorus is not present in the atmosphere as a gas. Instead, most phosphorus in the ecosystem exists as compounds, such as phosphate ions (PO43-), found in soil, water, sediment and rocks. Phosphorus is often a limiting nutrient (i.e., in short supply). Consequently, phosphorus is added to most agricultural fertilizers, which can cause environmental problems related to runoff in aquatic ecosystems.