Ensamblaje de plantillas dentro de plantillas de microsferas nanoestructuradas para cromatografía de alto rendimiento

Concurrent precision architecture of morphology and nanostructure in mesoporous microspheres is pivotal for high performance separations. Herein, we develop a template-in-template assembly nanostructuring (TiTAN) strategy to precisely synthesize monodisperse microspheres with ordered mesoporous nanostructure. Microfluidic droplet templating ensures uniform particle morphology (CV = 3%), while structure-directing agents within droplets enable tailored pore configurations, including 2D hexagonal, body-centered cubic, face-centered cubic, and cubic double gyroidal mesostructures. Through regulating hydrothermal conditions, structural parameters can be fined-tuned with 2 Å spatial resolution. By extending this manufacturing capability to a variety of material chemistries, chromatographic materials can now be de novo architectured with rationales, with the performance demonstrated by the solution of a classical separation challenge: resolving critical pairs (whose selectivity, α, infinitely approaching to 1), and with the shortest possible time. Beyond separation medium, the TiTAN strategy also suggests a route towards general synthesis of porous material with precision macroscopic morphology and microscopic nanostructure.