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Updated: Feb 6, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Ensamblaje del genoma a nivel de cromosoma del Gerbera (Gerbera hybrida) utilizando tecnologías de lectura larga HiFi
Yuta B Aoyagi1, Riki Shimada2, Hideki Hirakawa3
1Department of Frontier Research and Development, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
Abstract:
Gerbera hybrida is one of the most popular ornamental plants and also serves as a valuable model plant within the Asteraceae family. Here, we report both the nuclear and organellar genome assemblies and annotations of G. hybrida, which was developed through hybridization of two wild species. Sequencing was performed using a combination of PacBio high fidelity (HiFi) reads and chromatin capture reads (Omni-C). The total span of the nuclear genome assembly is 2.32 gigabases, and 99.3% of the sequence assembled into 25 scaffolds, consistent with the known chromosome number. Genome annotation of the nuclear genome identified 36,160 protein-coding genes and 11,572 non-coding transcripts. The mitochondrial genome had 363,511 bp and contains 36 protein-coding genes, 3 rRNAs, and 21 tRNAs, while the chloroplast genome is 151,898 bp in length and includes 85 protein-coding genes, 8 rRNAs, and 37 tRNAs. A syntenic analysis of the G. hybrida genome and published Asterales genomes demonstrated that Gerbera has undergone a whole-genome triplication. This reference genome provides a foundational resource for future molecular breeding and genetic research in Gerbera and the broader Asteraceae family.
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