Video Experimental Relacionado

Updated: Feb 6, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

Perfil de expresión génica identifica genes y vías relacionados con la ferroptosis en líneas celulares de cáncer de

M Balik-Meisner¹, D Phadke¹, D Mav¹

¹Sciome LLC, Durham, NC, United States.

Frontiers in molecular biosciences

|February 5, 2026

Resumen

La erastina induce la ferroptosis en células de cáncer colorrectal (CCR) alterando la expresión génica. Genes clave como ASNS y CHAC1 pueden servir como biomarcadores para estrategias de tratamiento personalizadas del CCR.

Área de la Ciencia:

Oncología
Biología Molecular
Bioquímica

Sus antecedentes:

El cáncer colorrectal (CCR) es una de las principales causas de muerte por cáncer en todo el mundo.
La resistencia a los medicamentos plantea un desafío importante en el tratamiento del CCR.
La ferroptosis, una vía de muerte celular dependiente del hierro, ofrece una nueva vía terapéutica.

Objetivo del estudio:

Investigar la respuesta transcripcional de las células de CCR a la erastina (ER).
Identificar genes y vías involucradas en la ferroptosis inducida por ER.
Explorar biomarcadores potenciales para la respuesta a la ferroptosis en el CCR.

Principales métodos:

Se realizó un análisis de expresión génica mediante microarrays en dos líneas celulares de CCR (HCT116 y HT-29).
Se analizaron los perfiles transcripcripcionales para identificar genes expresados diferencialmente tras el tratamiento con ER.
Se validaron los cambios en la expresión génica en una línea celular de CCR adicional (DLD-1).

Principales resultados:

Se observaron perfiles transcripcripcionales distintos entre las células HCT116 y HT-29.
Se enriquecieron comúnmente veintiséis transcritos en ambas líneas celulares tras el tratamiento con ER.
Genes como ASNS, PCK2, CHAC1 y DDIT4 se regularon significativamente al alza, lo que indica una respuesta ferroptótica conservada.

Conclusiones:

ASNS, CHAC1, PCK2, DDIT4 y ATF3/4 son biomarcadores potenciales para la ferroptosis en el CCR.
La monitorización de estas expresiones génicas podría ayudar a identificar pacientes que responden a los inductores de la ferroptosis.
Estos hallazgos respaldan el desarrollo de estrategias de tratamiento personalizadas para el CCR.

Palabras clave:

genes biomarcadores cáncer de colon erastina feroptosis análisis de vías

Más Videos Relacionados

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Published on: November 23, 2017

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization

Published on: October 3, 2025

Videos de Conceptos Relacionados

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Cell Specific Gene Expression

Cell Specific Gene Expression

What is Gene Expression?

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...

What is Gene Expression?

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...

mRNA Stability and Gene Expression

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability