Video Experimental Relacionado

Updated: Feb 7, 2026

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

Published on: January 4, 2018

Implicaciones del muestreo temporal en la microscopía de imagen de voltaje

Jakub Czuchnowski, Jerome Mertz

|February 6, 2026

Abstracto

Significance:

Voltage imaging microscopy has emerged as a powerful tool to investigate neural activity both in vivo and in vitro. Various imaging approaches have been developed, including point-scanning, line-scanning and wide-field microscopes, however the effects of their different temporal sampling methods on signal fidelity have not yet been fully investigated.

Aim:

To provide an analysis of the inherent advantages and disadvantages of temporal sampling in scanning and wide-field microscopes and their effect on the fidelity of voltage spike detection.

Approach:

We develop a mathematical framework based on a mixture of analytical modeling and computer simulations with Monte-Carlo approaches.

Results:

Scanning microscopes outperform wide-field microscopes in low signal-to-noise conditions and when only a small subset of spikes needs to be detected. Wide-field microscopes outperform scanning microscopes when the measurement is temporally undersampled and a large fraction of the spikes needs to be detected. Both modalities converge in performance as sampling increases and the frame rate reaches the decay rate of the voltage indicator.

Conclusions:

Our work provides guidance for the selection of optimal temporal sampling parameters for voltage imaging. Most importantly it advises against using scanning voltage imaging microscopes at frame rates below 500 Hz.

Más Videos Relacionados

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Miniaturized Sample Preparation for Transmission Electron Microscopy

Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

Videos de Conceptos Relacionados

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

Voltage

Voltage

The movement of electrons in a conductor requires some form of energy or work, usually provided by an external force, like a battery. This force is called the electromotive force or voltage. The voltage between two points, referred to as points "a" and "b," in an electric circuit is the energy (or work) needed to move a unit charge from point "a" to point "b," and this relationship is expressed mathematically as

Multiple Voltage Sources

Multiple Voltage Sources

Generally, a single battery is not enough to power some devices. In such cases, batteries can be combined in two ways: in series or in parallel.
In series, the positive terminal of one battery is connected to the negative terminal of another battery. Hence, the voltage of each battery is added to give the net voltage, which is increased because each battery boosts the electrons that enter it. The same current flows through each battery because they are connected in series.
Batteries are...

Voltage Dividers

Voltage Dividers

In electrical circuits, resistors can be connected in series, sequentially linked one after the other. In a series configuration, the same current flows through each resistor. Ohm's law is a fundamental principle to understand the behavior of resistors in series. It expresses the voltage across these resistors in terms of the current and resistance.
Kirchhoff's voltage law implies that the sum of the voltages across the resistors in series equals the source voltage. This means that the current...

Three-Phase Voltages

Three-Phase Voltages

A three-phase generator produces three voltages that are equal in magnitude but have a phase difference of 120 degrees. This identical magnitude and equal phase separated voltages are known as the balanced voltages and help to minimize power loss while ensuring a steady delivery of energy to connected loads. As voltage sources in a three-phase system can be configured in a wye or a delta formation, the loads connected to these systems can also be arranged in either configuration. This...